Co-Authors:
Shapiro, D.I., Department of Nematology, Volcani Center, Bet Dagan, 50-250, Israel, Integrated BioControl Systems, Inc., Lawrenceburg, IN 47025, United States
Glazer, I., Department of Nematology, Volcani Center, Bet Dagan, 50-250, Israel
Segal, D., Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel
Abstract:
Genetic variation in laboratory reared biocontrol agents may be reduced due to founder effect, inbreeding, and selection. We used random amplified polymorphic DNA (RAPD-PCR) to compare genetic variation in two strains of Heterorhabditis bacteriophora. One strain (IS5) was recently isolated from the field and the other strain (HP88) has been reared under laboratory conditions for over 10 years. For each strain, fifteen inbred lines were generated by eight cycles of selfing of a single hermaphrodite (reaching > 90 % homozygosity). Genomic DNA from each of the inbred lines was screened with fourteen decamer primers. Genetic variation was calculated based on average percentage similarity of DNA banding patterns and cluster analysis. The level of within population variation detected did not differ significantly between the two strains.
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