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פותח על ידי קלירמאש פתרונות בע"מ -
The genes involved in cytokinin biosynthesis in Erwinia herbicola pv. gypsophilae: Characterization and role in gall formation
Year:
1995
Source of publication :
Journal of Bacteriology
Authors :
ולינסקי, לאה
;
.
ליכטר, אמנון
;
.
מנוליס-ששון, שולמית
;
.
Volume :
177
Co-Authors:

Lichter, A.
Barash, Isaac
Valinsky, L., Department of Plant Pathology, ARO Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Manulis, S., Department of Plant Pathology, ARO Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel

Facilitators :
From page:
4457
To page:
4465
(
Total pages:
9
)
Abstract:
A locus conferring cytokinin production was previously isolated from the gall-forming bacterium Erwinia herbicola pv. gypsophilae. This locus resided in a cluster with the genes specifying indole-3-acetic acid production on the pathogenicity-associated plasmid pPATH (A. Lichter, S. Manulis, O. Sagee, Y. Gafni, J. Gray, R. Meilen, R. O. Morris, and I. Barash, Mol. Plant Microbe Interact., 8:114-121, 1995). Sequence analysis of this locus indicated the presence of a cytokinin biosynthesis gene (etz) homologous to other described cytokinin biosynthesis genes. A unique open reading frame (pre-etz) encoding 169 amino acids preceded etz and together with etz formed a region with a distinctive low G+C content. Northern (RNA) analysis indicated the presence of an etz-specific transcript of 1 kb and a common transcript for pre-etz and etz of 1.4 kb. The level of the 1-kb transcript was high in the late logarithmic phase and very low in the stationary phase. In contrast, the level of the 1.4-kb transcript was lower than that of the 1-kb transcript in the late logarithmic phase and predominant in the stationary phase. A marker exchange mutant of etz which did not produce cytokinins exhibited a reduction in gall size on Gypsophila cuttings and almost abolished disease symptoms in a whole-plant assay. Complementation of this marker exchange mutant with the intact etz gene on a multicopy plasmid resulted in overproduction of cytokinins and larger plant galls from which small shoots emerged. Insertional mutation in pre-etz resulted in a sharp decrease in both the level of the etz-specific transcript and cytokinin production. A frameshift mutation in pre-etz caused a similar reduction in the cytokinin level. A marker exchange mutation in pre-etz caused a reduction of symptoms but to a lower degree than the etz mutation. In the former mutant, cytokinin production and pathogenicity could not be restored by complementation. Furthermore, attempts to complement the etz marker exchange mutant with a plasmid containing an intact etz gene and a frameshift mutation in the pre- etz gene were unsuccessful. These results suggest that the mutations in pre- etz were trans dominant.
Note:
Related Files :
Base Sequence
cytokinins
Erwinia
frameshift mutation
Plant Diseases
Plants
Sequence Homology, Amino Acid
virulence
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29959
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:50
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Scientific Publication
The genes involved in cytokinin biosynthesis in Erwinia herbicola pv. gypsophilae: Characterization and role in gall formation
177

Lichter, A.
Barash, Isaac
Valinsky, L., Department of Plant Pathology, ARO Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Manulis, S., Department of Plant Pathology, ARO Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel

The genes involved in cytokinin biosynthesis in Erwinia herbicola pv. gypsophilae: Characterization and role in gall formation
A locus conferring cytokinin production was previously isolated from the gall-forming bacterium Erwinia herbicola pv. gypsophilae. This locus resided in a cluster with the genes specifying indole-3-acetic acid production on the pathogenicity-associated plasmid pPATH (A. Lichter, S. Manulis, O. Sagee, Y. Gafni, J. Gray, R. Meilen, R. O. Morris, and I. Barash, Mol. Plant Microbe Interact., 8:114-121, 1995). Sequence analysis of this locus indicated the presence of a cytokinin biosynthesis gene (etz) homologous to other described cytokinin biosynthesis genes. A unique open reading frame (pre-etz) encoding 169 amino acids preceded etz and together with etz formed a region with a distinctive low G+C content. Northern (RNA) analysis indicated the presence of an etz-specific transcript of 1 kb and a common transcript for pre-etz and etz of 1.4 kb. The level of the 1-kb transcript was high in the late logarithmic phase and very low in the stationary phase. In contrast, the level of the 1.4-kb transcript was lower than that of the 1-kb transcript in the late logarithmic phase and predominant in the stationary phase. A marker exchange mutant of etz which did not produce cytokinins exhibited a reduction in gall size on Gypsophila cuttings and almost abolished disease symptoms in a whole-plant assay. Complementation of this marker exchange mutant with the intact etz gene on a multicopy plasmid resulted in overproduction of cytokinins and larger plant galls from which small shoots emerged. Insertional mutation in pre-etz resulted in a sharp decrease in both the level of the etz-specific transcript and cytokinin production. A frameshift mutation in pre-etz caused a similar reduction in the cytokinin level. A marker exchange mutation in pre-etz caused a reduction of symptoms but to a lower degree than the etz mutation. In the former mutant, cytokinin production and pathogenicity could not be restored by complementation. Furthermore, attempts to complement the etz marker exchange mutant with a plasmid containing an intact etz gene and a frameshift mutation in the pre- etz gene were unsuccessful. These results suggest that the mutations in pre- etz were trans dominant.
Scientific Publication
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