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Plant Cell

Guterman, I., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Shalit, M., ARO, Neve Ya'ar, Israel
Menda, N., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Piestun, D., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Dafny-Yelin, M., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Shalev, G., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Bar, E., ARO, Neve Ya'ar, Israel
Davydov, O., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Ovadis, M., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Emanuel, M., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Wang, J., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Adam, Z., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Pichersky, E., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lewinsohn, E., ARO, Neve Ya'ar, Israel
Zamir, D., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Vainstein, A., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Weiss, D., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel

For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.
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Rose scent: Genomics approach to discovering novel floral fragrance-related genes
14

Guterman, I., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Shalit, M., ARO, Neve Ya'ar, Israel
Menda, N., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Piestun, D., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Dafny-Yelin, M., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Shalev, G., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Bar, E., ARO, Neve Ya'ar, Israel
Davydov, O., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Ovadis, M., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Emanuel, M., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Wang, J., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Adam, Z., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Pichersky, E., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lewinsohn, E., ARO, Neve Ya'ar, Israel
Zamir, D., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Vainstein, A., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Weiss, D., Inst. of Plant Sci./Genet. in Agric., Faculty of Agricultural Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel

Rose scent: Genomics approach to discovering novel floral fragrance-related genes
For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.
Scientific Publication
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