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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Influence of Effluent Irrigation on Community Composition and Function of Ammonia-Oxidizing Bacteria in Soil
Year:
2001
Authors :
מינץ, דרור
;
.
מנדלבאום, רפי
;
.
עובד, תמר
;
.
Volume :
67
Co-Authors:
Oved, T., Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, Bet-Dagan 50-250, Israel, Water-Soil-Environment, Agricultural Engineering, Technion Institute, Haifa 32-000, Israel
Shaviv, A., Water-Soil-Environment, Agricultural Engineering, Technion Institute, Haifa 32-000, Israel
Goldrath, T., Water-Soil-Environment, Agricultural Engineering, Technion Institute, Haifa 32-000, Israel
Mandelbaum, R.T., Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, Bet-Dagan 50-250, Israel
Minz, D., Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, Bet-Dagan 50-250, Israel, Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, P.O. Box 06, Bet-Dagan 50-250, Israel
Facilitators :
From page:
3426
To page:
3433
(
Total pages:
8
)
Abstract:
The effect of effluent irrigation on community composition and function of ammonia-oxidizing bacteria (AOB) in soil was evaluated, using techniques of molecular biology and analytical soil chemistry. Analyses were conducted on soil sampled from lysimeters and from a grapefruit orchard which had been irrigated with wastewater effluent or fertilizer-amended water (FAW). Specifically, comparisons of AOB community composition were conducted using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of the gene encoding the α-subunit of the ammonia monooxygenase gene (amoA) recovered from soil samples and subsequent sequencing of relevant bands. A significant and consistent shift in the population composition of AOB was detected in soil irrigated with effluent. This shift was absent in soils irrigated with FAW, despite the fact that the ammonium concentration in the FAW was similar. At the end of the irrigation period, Nitrosospira-like populations were dominant in soils irrigated with FAW, while Nitrosomonas-like populations were dominant in effluent-irrigated soils. Furthermore, DGGE analysis of the amoA gene proved to be a powerful tool in evaluating the soil AOB community population and population shifts therein.
Note:
Related Files :
Agriculture
bacteria
Electrophoresis
Genetics
metabolism
Microbiology
molecular genetics
Oxidoreductases
soil
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.67.8.3426-3433.2001
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30045
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Influence of Effluent Irrigation on Community Composition and Function of Ammonia-Oxidizing Bacteria in Soil
67
Oved, T., Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, Bet-Dagan 50-250, Israel, Water-Soil-Environment, Agricultural Engineering, Technion Institute, Haifa 32-000, Israel
Shaviv, A., Water-Soil-Environment, Agricultural Engineering, Technion Institute, Haifa 32-000, Israel
Goldrath, T., Water-Soil-Environment, Agricultural Engineering, Technion Institute, Haifa 32-000, Israel
Mandelbaum, R.T., Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, Bet-Dagan 50-250, Israel
Minz, D., Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, Bet-Dagan 50-250, Israel, Inst. Soil, Water and Environ. Sci., Agricultural Research Organization, Volcani Research Center, P.O. Box 06, Bet-Dagan 50-250, Israel
Influence of Effluent Irrigation on Community Composition and Function of Ammonia-Oxidizing Bacteria in Soil
The effect of effluent irrigation on community composition and function of ammonia-oxidizing bacteria (AOB) in soil was evaluated, using techniques of molecular biology and analytical soil chemistry. Analyses were conducted on soil sampled from lysimeters and from a grapefruit orchard which had been irrigated with wastewater effluent or fertilizer-amended water (FAW). Specifically, comparisons of AOB community composition were conducted using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of the gene encoding the α-subunit of the ammonia monooxygenase gene (amoA) recovered from soil samples and subsequent sequencing of relevant bands. A significant and consistent shift in the population composition of AOB was detected in soil irrigated with effluent. This shift was absent in soils irrigated with FAW, despite the fact that the ammonium concentration in the FAW was similar. At the end of the irrigation period, Nitrosospira-like populations were dominant in soils irrigated with FAW, while Nitrosomonas-like populations were dominant in effluent-irrigated soils. Furthermore, DGGE analysis of the amoA gene proved to be a powerful tool in evaluating the soil AOB community population and population shifts therein.
Scientific Publication