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פותח על ידי קלירמאש פתרונות בע"מ -
Defined medium supporting development of cleansed Plasmodium berghei ookinetes in Anopheles stephensi
Year:
1992
Authors :
סמיש, מיכאל
;
.
Volume :
22
Co-Authors:
Samish, M., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Yano, K., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Kozlowska, A., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Maramorosch, K., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Facilitators :
From page:
15
To page:
22
(
Total pages:
8
)
Abstract:
Hamsters blood infected with Plasmodium berghei was cultured in vitro for the development of ookinetes. The ookinetes were separated from blood components, suspended in various defined media and fed to Anopheles stephensi through a membrane. The development of the oocysts and infective sporozoites was recorded. Mosquitoes infected with ookinetes suspended i L15 formulated into L15-B, L15-D (a medium specially modified for this purpose), IPL-41 or 199 media with no proteins added, developed at least as many oocysts as the control mosquitoes fed ookinetes suspended in blood. Ookinetes suspended in the L15-B medium yielded more oocysts than after feeding ookinetes suspended in L15-B with 5% casein. Sporozoites from mosquitoes maintained on blood, L15-B, L15-D, or L15-B with 5% casein were shown to be infective to hamsters. Mosquitoes fed ookinetes suspended in sucrose solutions showed very few oocysts, but the yield was increased when a blood meal was given 2-4 days after the infective meal. Some of the oocysts which had developed from the ookinetes suspended in artificial media were found to have degenerated. The described system could be potentially useful for a study of the interaction between the vector physiology and the parasite. The possible use of the system to learn which media should be developed in the future for in vitro cultivation of oocysts is discussed. © 1992.
Note:
Related Files :
Animal
animal experiment
artificial feeding of Anopheles stephensi
Female
ookinetes of Plasmodium berghei
parasite development
Plasmodium berghei
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0020-7519(92)90074-U
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30098
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
Defined medium supporting development of cleansed Plasmodium berghei ookinetes in Anopheles stephensi
22
Samish, M., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Yano, K., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Kozlowska, A., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Maramorosch, K., Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, NJ 08903, United States
Defined medium supporting development of cleansed Plasmodium berghei ookinetes in Anopheles stephensi
Hamsters blood infected with Plasmodium berghei was cultured in vitro for the development of ookinetes. The ookinetes were separated from blood components, suspended in various defined media and fed to Anopheles stephensi through a membrane. The development of the oocysts and infective sporozoites was recorded. Mosquitoes infected with ookinetes suspended i L15 formulated into L15-B, L15-D (a medium specially modified for this purpose), IPL-41 or 199 media with no proteins added, developed at least as many oocysts as the control mosquitoes fed ookinetes suspended in blood. Ookinetes suspended in the L15-B medium yielded more oocysts than after feeding ookinetes suspended in L15-B with 5% casein. Sporozoites from mosquitoes maintained on blood, L15-B, L15-D, or L15-B with 5% casein were shown to be infective to hamsters. Mosquitoes fed ookinetes suspended in sucrose solutions showed very few oocysts, but the yield was increased when a blood meal was given 2-4 days after the infective meal. Some of the oocysts which had developed from the ookinetes suspended in artificial media were found to have degenerated. The described system could be potentially useful for a study of the interaction between the vector physiology and the parasite. The possible use of the system to learn which media should be developed in the future for in vitro cultivation of oocysts is discussed. © 1992.
Scientific Publication
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