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Phytoparasitica
Antignus, Y., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Lachman, O., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Pearlsman, M., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gofman, R., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Bar-Joseph, M., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Using the sequential PAGE method for detection of small circular RNA molecules we isolated a viroid from greenhouse-grown tomato plants exhibiting severe stunting in Israel. The viroid was transmitted to tomato and to several other solanaceous plants by graft and mechanical inoculation, but only tomato plants showed symptoms of disease. Cloning and sequencing revealed that the viroid RNA is composed of 363 nucleotides, has 92% identity with the type strain (Ivory Coast strain) of Tomato apical stunt viroid (TASVd) and 99% identity with the Indonesian strain of this viroid. The experimental host range of TASVd-Is differs significantly from that of the type strain of TASVd. The possible epidemiological consequences leading to TASVd spread in geographically distant areas are discussed.
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תנאי שימוש
A new disease of greenhouse tomatoes in Israel caused by a distinct strain of Tomato apical stunt viroid (TASVd)
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Antignus, Y., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Lachman, O., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Pearlsman, M., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gofman, R., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Bar-Joseph, M., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
A new disease of greenhouse tomatoes in Israel caused by a distinct strain of Tomato apical stunt viroid (TASVd)
Using the sequential PAGE method for detection of small circular RNA molecules we isolated a viroid from greenhouse-grown tomato plants exhibiting severe stunting in Israel. The viroid was transmitted to tomato and to several other solanaceous plants by graft and mechanical inoculation, but only tomato plants showed symptoms of disease. Cloning and sequencing revealed that the viroid RNA is composed of 363 nucleotides, has 92% identity with the type strain (Ivory Coast strain) of Tomato apical stunt viroid (TASVd) and 99% identity with the Indonesian strain of this viroid. The experimental host range of TASVd-Is differs significantly from that of the type strain of TASVd. The possible epidemiological consequences leading to TASVd spread in geographically distant areas are discussed.
Scientific Publication
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