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פותח על ידי קלירמאש פתרונות בע"מ -
Synthesis and secretion of the mouse whey acidic protein in transgenic sheep
Year:
1996
Source of publication :
Transgenic Research
Authors :
שמאי, אבי
;
.
Volume :
5
Co-Authors:
Wall, R.J., Gene Eval. and Mapping Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
Rexroad Jr., C.E., Gene Eval. and Mapping Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
Powell, A., Gene Eval. and Mapping Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
Shamay, A., Lab. of Biochemistry and Metabolism, Natl. Inst. Diabet., Digest. K., National Institutes of Health, Bethesda, MD 20892, United States
McKnight, R., Lab. of Biochemistry and Metabolism, Natl. Inst. Diabet., Digest. K., National Institutes of Health, Bethesda, MD 20892, United States
Hennighausen, L., Lab. of Biochemistry and Metabolism, Natl. Inst. Diabet., Digest. K., National Institutes of Health, Bethesda, MD 20892, United States
Facilitators :
From page:
67
To page:
72
(
Total pages:
6
)
Abstract:
The synthesis of foreign proteins can be targeted to the mammary gland of transgenic animals, thus permitting commercial purification of otherwise unavailable proteins from milk. Genetic regulatory elements from the mouse whey acidic protein (WAP) gene have been used successfully to direct expression of transgenes to the mammary gland of mice, goats and pigs. To extend the practical usefulness of WAP promoter-driven fusion genes and further characterize WAP expression in heterologous species, we introduced a 6.8 kb DNA fragment containing the genomic form of the mouse WAP gene into sheep zygotes. Two lines of transgenic sheep were produced. The transgene was expressed in mammary tissue of both lines and intact WAP was secreted into milk at concentrations estimated to range from 100 to 500 mg/litre. Ectopic WAP gene expression was found in salivary gland, spleen, liver, lung, heart muscle, kidney and bone marrow of one founder ewe. WAP RNA was not detected in skeletal muscle and intestine. These data suggest that unlike pigs, sheep may possess nuclear factors in a variety of tissues that interact with WAP regulatory sequences. Though the data presented are based on only two lines, these findings suggest WAP regulatory sequences may not be suitable as control elements for transgenes in sheep bioreactors.
Note:
Related Files :
Animal
Animals
biosynthesis
Female
Genetics
metabolism
mice
milk
sheep
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30107
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
Synthesis and secretion of the mouse whey acidic protein in transgenic sheep
5
Wall, R.J., Gene Eval. and Mapping Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
Rexroad Jr., C.E., Gene Eval. and Mapping Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
Powell, A., Gene Eval. and Mapping Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
Shamay, A., Lab. of Biochemistry and Metabolism, Natl. Inst. Diabet., Digest. K., National Institutes of Health, Bethesda, MD 20892, United States
McKnight, R., Lab. of Biochemistry and Metabolism, Natl. Inst. Diabet., Digest. K., National Institutes of Health, Bethesda, MD 20892, United States
Hennighausen, L., Lab. of Biochemistry and Metabolism, Natl. Inst. Diabet., Digest. K., National Institutes of Health, Bethesda, MD 20892, United States
Synthesis and secretion of the mouse whey acidic protein in transgenic sheep
The synthesis of foreign proteins can be targeted to the mammary gland of transgenic animals, thus permitting commercial purification of otherwise unavailable proteins from milk. Genetic regulatory elements from the mouse whey acidic protein (WAP) gene have been used successfully to direct expression of transgenes to the mammary gland of mice, goats and pigs. To extend the practical usefulness of WAP promoter-driven fusion genes and further characterize WAP expression in heterologous species, we introduced a 6.8 kb DNA fragment containing the genomic form of the mouse WAP gene into sheep zygotes. Two lines of transgenic sheep were produced. The transgene was expressed in mammary tissue of both lines and intact WAP was secreted into milk at concentrations estimated to range from 100 to 500 mg/litre. Ectopic WAP gene expression was found in salivary gland, spleen, liver, lung, heart muscle, kidney and bone marrow of one founder ewe. WAP RNA was not detected in skeletal muscle and intestine. These data suggest that unlike pigs, sheep may possess nuclear factors in a variety of tissues that interact with WAP regulatory sequences. Though the data presented are based on only two lines, these findings suggest WAP regulatory sequences may not be suitable as control elements for transgenes in sheep bioreactors.
Scientific Publication
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