אסיף מאגר המחקר החקלאי

Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e

Year:

1974

Source of publication :

Authors :

Volume :

14

Co-Authors:

Lavi, U., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Nattenberg, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Ronen, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Marcus, M., Dept. Genet., Hebrew Univ., Jerusalem, Israel

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Abstract:

The virulent phage phie of B. subtilis which contains hydroxymethyluracil in its DNA requires host DNA polymerase III for its DNA replication. DNA polymerase III(ts) mutant cells infected with phi epsilon at restrictive temperatures do not support phage DNA synthesis. However, phi epsilon grows normally at low and high temperatures in the mutant's parent strain and in spontaneous DNA polymerase III+ revertants isolated from the mutant strain. Temperature shift down experiments with phi epsilon infected cells having thermosensitive DNA polymerase III (pol III(ts)) indicate that at 48 C the thermolabile DNA polymerase III is irreversibly inactivated and has to be synthesized de novo after the shift to 37 C, before phage DNA synthesis can begin. Temperature shift up experiments with phi epsilon infected mutant cells show that phage replication is arrested immediately after the temperature shift and indicate that phi epsilon requires DNA polymerase III throughout its replication stage.

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