נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Global transcriptional analysis of dehydrated Salmonella enterica serovar Typhimurium
Year:
2012
Authors :
גרוזדב, נדיה
;
.
סלע, שלמה
;
.
עפאים, שני
;
.
פינטו, רבקה
;
.
Volume :
78
Co-Authors:
Gruzdev, N., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel
Mcclelland, M., Vaccine Research Institute of San Diego, San Diego, CA, United States, Department of Pathology and Laboratory Medicine, University of California, Irvine, CA, United States
Porwollik, S., Vaccine Research Institute of San Diego, San Diego, CA, United States, Department of Pathology and Laboratory Medicine, University of California, Irvine, CA, United States
Ofaim, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel
Saldinger-Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
7866
To page:
7875
(
Total pages:
10
)
Abstract:
Despite the scientific and industrial importance of desiccation tolerance in Salmonella, knowledge regarding its genetic basis is still scarce. In the present study, we performed a transcriptomic analysis of dehydrated and water-suspended Salmonella enterica serovar Typhimurium using microarrays. Dehydration induced expression of 90 genes and downregulated that of 7 genes. Ribosomal structural genes represented the most abundant functional group with a relatively higher transcription during dehydration. Other main induced functional groups included genes involved in amino acid metabolism, energy production, ion transport, transcription, and stress response. The highest induction was observed in the kdpFABC operon, encoding a potassium transport channel. Knockout mutations were generated in nine upregulated genes. Five mutants displayed lower tolerance to desiccation, implying the involvement of the corresponding genes in the adaptation of Salmonella to desiccation. These included genes encoding the isocitrate-lyase AceA, the lipid A biosynthesis palmitoleoyl-acyltransferase Ddg, the modular iron-sulfur cluster scaffolding protein NifU, the global regulator Fnr, and the alternative sigma factor RpoE. Notably, these proteins were previously implicated in the response of Salmonella to oxidative stress, heat shock, and cold shock. A strain with a mutation in the structural gene kdpA had a tolerance to dehydration comparable to that of the parent strain, implying that potassium transport through this system is dispensable for early adaptation to the dry environment. Nevertheless, this mutant was significantly impaired in long-term persistence during cold storage. Our findings indicate the involvement of a relatively small fraction of the Salmonella genome in transcriptional adjustment from water to dehydration, with a high prevalence of genes belonging to the protein biosynthesis machinery. © 2012, American Society for Microbiology.
Note:
Related Files :
Amino Acids
Biochemistry
biosynthesis
cold storage
Genetics
metabolism
mutation
proteins
Salmonella
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.01822-12
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30131
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
Scientific Publication
Global transcriptional analysis of dehydrated Salmonella enterica serovar Typhimurium
78
Gruzdev, N., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel
Mcclelland, M., Vaccine Research Institute of San Diego, San Diego, CA, United States, Department of Pathology and Laboratory Medicine, University of California, Irvine, CA, United States
Porwollik, S., Vaccine Research Institute of San Diego, San Diego, CA, United States, Department of Pathology and Laboratory Medicine, University of California, Irvine, CA, United States
Ofaim, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel
Saldinger-Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel
Global transcriptional analysis of dehydrated Salmonella enterica serovar Typhimurium
Despite the scientific and industrial importance of desiccation tolerance in Salmonella, knowledge regarding its genetic basis is still scarce. In the present study, we performed a transcriptomic analysis of dehydrated and water-suspended Salmonella enterica serovar Typhimurium using microarrays. Dehydration induced expression of 90 genes and downregulated that of 7 genes. Ribosomal structural genes represented the most abundant functional group with a relatively higher transcription during dehydration. Other main induced functional groups included genes involved in amino acid metabolism, energy production, ion transport, transcription, and stress response. The highest induction was observed in the kdpFABC operon, encoding a potassium transport channel. Knockout mutations were generated in nine upregulated genes. Five mutants displayed lower tolerance to desiccation, implying the involvement of the corresponding genes in the adaptation of Salmonella to desiccation. These included genes encoding the isocitrate-lyase AceA, the lipid A biosynthesis palmitoleoyl-acyltransferase Ddg, the modular iron-sulfur cluster scaffolding protein NifU, the global regulator Fnr, and the alternative sigma factor RpoE. Notably, these proteins were previously implicated in the response of Salmonella to oxidative stress, heat shock, and cold shock. A strain with a mutation in the structural gene kdpA had a tolerance to dehydration comparable to that of the parent strain, implying that potassium transport through this system is dispensable for early adaptation to the dry environment. Nevertheless, this mutant was significantly impaired in long-term persistence during cold storage. Our findings indicate the involvement of a relatively small fraction of the Salmonella genome in transcriptional adjustment from water to dehydration, with a high prevalence of genes belonging to the protein biosynthesis machinery. © 2012, American Society for Microbiology.
Scientific Publication
You may also be interested in