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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
A new aubergine disease caused by a whitefly-borne strain of Tomato mild mottle virus (TomMMoV)
Year:
2013
Source of publication :
Plant Pathology
Authors :
אנטיגנוס, יחזקאל
;
.
דומברובסקי, אביב
;
.
לכמן, עודד
;
.
פרלסמן, מלניה
;
.
Volume :
62
Co-Authors:
Dombrovsky, A., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Sapkota, R., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Lachman, O., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Pearlsman, M., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Antignus, Y., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
750
To page:
759
(
Total pages:
10
)
Abstract:
The aims of the present study were to further characterize the causal agent of a new viral disease of aubergines in Israel, first observed in 2003 and tentatively named eggplant mild leaf mottle virus (EMLMV) in a previous work, and to identify the vector responsible for its spread. The disease could be transmitted mechanically from infected source plants to healthy aubergines or laboratory test plants. Transmission electron microscopy (TEM) analysis of purified virus preparations indicated the presence of viral particles with a flexible filamentous morphology (approximately 720 nm long). TEM analysis of ultrathin sections prepared from infected leaf tissue revealed the presence of cytoplasmic inclusion bodies with pinwheel and crystalline structures, typical of those induced by potyviral infection. The viral coat protein subunit was shown to have a molecular weight of 37·5 kDa by SDS-PAGE analysis. The viral particles reacted positively in western blot analysis with an antiserum against Tomato mild mottle virus (TomMMoV) from Yemen, described as a potyvirus, vectored by the aphid Myzus persicae. The current study describes some biological properties of EMLMV and presents evidence for its transmission by the whitefly Bemisia tabaci, but not by three aphid species. The taxonomic relationship between EMLMV and TomMMoV is discussed based on their biological characteristics and sequence analysis of their genomes. It is suggested that the Israeli EMLMV should be considered a distant strain of TomMMoV, designated TomMMoV-IL, according to the present rules of Potyviridae molecular taxonomy. © 2012 British Society for Plant Pathology.
Note:
Related Files :
Aleyrodidae
Aphididae
Bemisia tabaci
cytoplasm
Israel
molecular analysis
Myzus persicae
Solanum melongena
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/ppa.12004
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30135
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
A new aubergine disease caused by a whitefly-borne strain of Tomato mild mottle virus (TomMMoV)
62
Dombrovsky, A., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Sapkota, R., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Lachman, O., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Pearlsman, M., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Antignus, Y., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, 50250, Israel
A new aubergine disease caused by a whitefly-borne strain of Tomato mild mottle virus (TomMMoV)
The aims of the present study were to further characterize the causal agent of a new viral disease of aubergines in Israel, first observed in 2003 and tentatively named eggplant mild leaf mottle virus (EMLMV) in a previous work, and to identify the vector responsible for its spread. The disease could be transmitted mechanically from infected source plants to healthy aubergines or laboratory test plants. Transmission electron microscopy (TEM) analysis of purified virus preparations indicated the presence of viral particles with a flexible filamentous morphology (approximately 720 nm long). TEM analysis of ultrathin sections prepared from infected leaf tissue revealed the presence of cytoplasmic inclusion bodies with pinwheel and crystalline structures, typical of those induced by potyviral infection. The viral coat protein subunit was shown to have a molecular weight of 37·5 kDa by SDS-PAGE analysis. The viral particles reacted positively in western blot analysis with an antiserum against Tomato mild mottle virus (TomMMoV) from Yemen, described as a potyvirus, vectored by the aphid Myzus persicae. The current study describes some biological properties of EMLMV and presents evidence for its transmission by the whitefly Bemisia tabaci, but not by three aphid species. The taxonomic relationship between EMLMV and TomMMoV is discussed based on their biological characteristics and sequence analysis of their genomes. It is suggested that the Israeli EMLMV should be considered a distant strain of TomMMoV, designated TomMMoV-IL, according to the present rules of Potyviridae molecular taxonomy. © 2012 British Society for Plant Pathology.
Scientific Publication
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