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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains
Year:
1997
Source of publication :
Journal of General Virology
Authors :
גל-און, עמית
;
.
רקח, בנימין
;
.
Volume :
78
Co-Authors:
Desbiez, C., Stn. de Pathol. Veg., INRA, Domaine Saint Maurice, 84143 Montfavet Cedex, France
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Raccah, B., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Lecoq, H., Stn. de Pathol. Veg., INRA, Domaine Saint Maurice, 84143 Montfavet Cedex, France
Facilitators :
From page:
2073
To page:
2076
(
Total pages:
4
)
Abstract:
Monoclonal antibodies (MAbs) raised against the coat protein of zucchini yellow mosaic potyviros (ZYMV) were characterized by epitope mapping using synthetic oligopeptides. Two mutant viruses with a mutation in the amino acid sequence important for epitope recognition in vitro were obtained by site-directed mutagenesis of a full-length cDNA of ZYMV. Two MAbs, CC11 and DD2, could distinguish specifically between these mutants in mixed infections, or after sequential inoculations of muskmelons. Sequential inoculations of the mutants and analysis with MAbs CC11 and DD2 revealed that cross-protection was established between these quasi-isogenic strains within 48 h.
Note:
Related Files :
Antibodies, Monoclonal
Base Sequence
Epitope mapping
Mosaic virus
Peptide Fragments
vegetables
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30141
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
Characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains
78
Desbiez, C., Stn. de Pathol. Veg., INRA, Domaine Saint Maurice, 84143 Montfavet Cedex, France
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Raccah, B., Department of Virology, Agricultural Research Organization, Volcani Center, POB 6, Bet Dagan 50250, Israel
Lecoq, H., Stn. de Pathol. Veg., INRA, Domaine Saint Maurice, 84143 Montfavet Cedex, France
Characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains
Monoclonal antibodies (MAbs) raised against the coat protein of zucchini yellow mosaic potyviros (ZYMV) were characterized by epitope mapping using synthetic oligopeptides. Two mutant viruses with a mutation in the amino acid sequence important for epitope recognition in vitro were obtained by site-directed mutagenesis of a full-length cDNA of ZYMV. Two MAbs, CC11 and DD2, could distinguish specifically between these mutants in mixed infections, or after sequential inoculations of muskmelons. Sequential inoculations of the mutants and analysis with MAbs CC11 and DD2 revealed that cross-protection was established between these quasi-isogenic strains within 48 h.
Scientific Publication
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