אסיף מאגר המחקר החקלאי

Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.)

Year:

1994

Source of publication :

Authors :

Volume :

80

Co-Authors:

Lavi, U., Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Akkaya, M., Soybean and Alfalfa Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Center, Beltsville, 20705-2350, Maryland, United States
Bhagwat, A., Soybean and Alfalfa Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Center, Beltsville, 20705-2350, Maryland, United States
Lahav, E., Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Cregan, P.B., Soybean and Alfalfa Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Center, Beltsville, 20705-2350, Maryland, United States

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Abstract:

Generation of Simple Sequence Repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequences flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion. © 1994 Kluwer Academic Publishers.

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