Co-Authors:
Fridman, E., R. H. Smith Inst. Plant Sci./G. A., Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel, Dept. of Molec., Cell./Devmtl. Biol., University of Michigan, Ann Arbor, MI 48109-1048, United States
Carrari, F., Department Willmitzer, M.-Planck-Inst. Molec. Pfl. Physiol., Am Mühlenber 1, 14476 Golm, Germany
Liu, Y.-S., R. H. Smith Inst. Plant Sci./G. A., Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel, Lab. of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, United States
Fernie, A.R., Department Willmitzer, M.-Planck-Inst. Molec. Pfl. Physiol., Am Mühlenber 1, 14476 Golm, Germany
Zamir, D., R. H. Smith Inst. Plant Sci./G. A., Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel
Abstract:
To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (UN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.