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The Salmonella transcriptome in lettuce and cilantro soft rot reveals a niche overlap with the animal host intestine
Year:
2013
Authors :
סלע, שלמה
;
.
Volume :
79
Co-Authors:
Goudeau, D.M., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Parker, C.T., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Zhou, Y., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Sela, S., Microbial Food Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, ARO, The Volcani Center, Beth-Dagan, Israel
Kroupitski, Y., Microbial Food Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, ARO, The Volcani Center, Beth-Dagan, Israel
Brandl, M.T., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Facilitators :
From page:
250
To page:
262
(
Total pages:
13
)
Abstract:
Fresh vegetables have been recurrently associated with salmonellosis outbreaks, and Salmonella contamination of retail produce has been correlated positively with the presence of soft rot disease. We observed that population sizes of Salmonella enterica serovar Typhimurium SL1344 increased 56-fold when inoculated alone onto cilantro leaves, versus 2,884-fold when coinoculated with Dickeya dadantii, a prevalent pathogen that macerates plant tissue. A similar trend in S. enterica populations was observed for soft-rotted lettuce leaves. Transcriptome analysis of S. enterica cells that colonized D. dadantii-infected lettuce and cilantro leaves revealed a clear shift toward anaerobic metabolism and catabolism of substrates that are available due to the degradation of plant cells by the pectinolytic pathogen. Twenty-nine percent of the genes that were upregulated in cilantro macerates were also previously observed to have increased expression levels in the chicken intestine. Furthermore, multiple genes induced in soft rot lesions are also involved in the colonization of mouse, pig, and bovine models of host infection. Among those genes, the operons for ethanolamine and propanediol utilization as well as for the synthesis of cobalamin, a cofactor in these pathways, were the most highly upregulated genes in lettuce and cilantro lesions. In S. Typhimurium strain LT2, population sizes of mutants deficient in propanediol utilization or cobalamin synthesis were 10- and 3-fold lower, respectively, than those of the wildtype strain in macerated cilantro (P<0.0002); in strain SL1344, such mutants behaved similarly to the parental strain. Anaerobic conditions and the utilization of nutrients in macerated plant tissue that are also present in the animal intestine indicate a niche overlap that may explain the high level of adaptation of S. enterica to soft rot lesions, a common postharvest plant disease. © 2013, American Society for Microbiology.
Note:
Related Files :
Animal
Animals
cattle
gene expression
Genetics
metabolism
mice
Salmonella
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.02290-12
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30335
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
Scientific Publication
The Salmonella transcriptome in lettuce and cilantro soft rot reveals a niche overlap with the animal host intestine
79
Goudeau, D.M., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Parker, C.T., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Zhou, Y., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
Sela, S., Microbial Food Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, ARO, The Volcani Center, Beth-Dagan, Israel
Kroupitski, Y., Microbial Food Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, ARO, The Volcani Center, Beth-Dagan, Israel
Brandl, M.T., Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States
The Salmonella transcriptome in lettuce and cilantro soft rot reveals a niche overlap with the animal host intestine
Fresh vegetables have been recurrently associated with salmonellosis outbreaks, and Salmonella contamination of retail produce has been correlated positively with the presence of soft rot disease. We observed that population sizes of Salmonella enterica serovar Typhimurium SL1344 increased 56-fold when inoculated alone onto cilantro leaves, versus 2,884-fold when coinoculated with Dickeya dadantii, a prevalent pathogen that macerates plant tissue. A similar trend in S. enterica populations was observed for soft-rotted lettuce leaves. Transcriptome analysis of S. enterica cells that colonized D. dadantii-infected lettuce and cilantro leaves revealed a clear shift toward anaerobic metabolism and catabolism of substrates that are available due to the degradation of plant cells by the pectinolytic pathogen. Twenty-nine percent of the genes that were upregulated in cilantro macerates were also previously observed to have increased expression levels in the chicken intestine. Furthermore, multiple genes induced in soft rot lesions are also involved in the colonization of mouse, pig, and bovine models of host infection. Among those genes, the operons for ethanolamine and propanediol utilization as well as for the synthesis of cobalamin, a cofactor in these pathways, were the most highly upregulated genes in lettuce and cilantro lesions. In S. Typhimurium strain LT2, population sizes of mutants deficient in propanediol utilization or cobalamin synthesis were 10- and 3-fold lower, respectively, than those of the wildtype strain in macerated cilantro (P<0.0002); in strain SL1344, such mutants behaved similarly to the parental strain. Anaerobic conditions and the utilization of nutrients in macerated plant tissue that are also present in the animal intestine indicate a niche overlap that may explain the high level of adaptation of S. enterica to soft rot lesions, a common postharvest plant disease. © 2013, American Society for Microbiology.
Scientific Publication
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