אסיף מאגר המחקר החקלאי

The effect of under- and overexpressed CoEXGI-encoded exoglucanase secreted by Candida oleophila on the biocontrol of Penicillium digitatum

Year:

2003

Source of publication :

Authors :

Volume :

20

Co-Authors:

Yehuda, H., Department of Molecular Genetics, MIGAL Galilee Technology Center, PO Box 831, Kiryat-Shmona 11016, Israel
Droby, S., Department of Postharvest Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Bar-Shimon, M., Department of Postharvest Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Wisniewski, M., Appalachian Fruit Research Station, USDA - ARS, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Goldway, M., Department of Molecular Genetics, MIGAL Galilee Technology Center, PO Box 831, Kiryat-Shmona 11016, Israel

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Abstract:

The yeast, Candida oleophila, is acknowledged for its biocontrol activity against phostharvest moulds. However, the mechanism of this activity is not fully understood. One of the conjectured modes of action is associated with extracellular lytic enzymes, such as β-exoglucanase. The relationship of β-exoglucanase in the biocontrol activity of C. oleophila was investigated by generating C. oleophila CoEXG1-knockouts and double-CoEXG1 transformants. The knockout transformants secreted 0-13% of the exoglucanase activity detected in the medium of the untransformed yeast (depending on the medium), indicating that CoEXG1 is the main gene responsible for the production of the secreted exoglucanase. Correspondingly, the double-CoEXG1 transformants secreted approximately twice as much 1,3-β-exoglucanase as the untransformed C. oleophila. The biocontrol activity of the CoEXG1-knockout and the double-CoEXG1 transformants against Penicillium digitatum did not differ from that of the untransformed C. oleophila on kumquats. These results imply that the 1,3-β-exoglucanase encoded by the gene CoEXG1 is not involved in the biocontrol activity of C. oleophila against P. digitatum under these experimental terms. However, these findings do not rule out the possibilities, that the participation of CoEXG1 in biocontrol is dependent on the activity of other gene products, or that its effect may be manifested under altered environmental conditions. Copyright © 2003 John Wiley & Sons, Ltd.

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