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פותח על ידי קלירמאש פתרונות בע"מ -
Dual regulation of βlactoglobulin/human serum albumin gene expression by the extracellular matrix in mammary cells from transgenic mice
Year:
1996
Source of publication :
Experimental Cell Research
Authors :
אילן, נטע
;
.
ברש, איתמר
;
.
פיירמן, אלכסנדר
;
.
שני, משה
;
.
Volume :
224
Co-Authors:
Ilan, N., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Barash, I., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Faerman, A., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Facilitators :
From page:
28
To page:
38
(
Total pages:
11
)
Abstract:
Mammary explants and epithelial cell cultures from transgenic mice carrying the human serum albumin (HSA) gene or minigenes behind the regulatory sequences of the ovine β-lactoglobulin gene were analyzed. Previously we demonstrated that mammary explants from virgin female transgenic mice synthesize and secrete high levels of HSA during the first day in culture. Here we present a detailed analysis of endogenous and transgene expression during the first 20 h of mammary explant cultures. We show that HSA genes as well as endogenous milk protein genes are rapidly induced upon explantation. Unexpectedly, HSA was synthesized also in mammary explants from strains that do not secrete HSA into the milk, indicating the existence of a cryptic potential to express the transgene. Histological examination revealed that some luminal epithelial cells detached from the underlying extracellular matrix (ECM) soon after explantation. Epithelial cell cultures from nonsecreting strains grown on plastic rapidly induced transgene expression and secreted higher levels of HSA into the medium compared to cells grown on collagen. These results suggest that tissue organization and most likely the interaction of epithelial cells with the ECM are intimately involved in the control of HSA transgene expression. © 1998 Academic Press, Inc.
Note:
Related Files :
Animals
animal tissue
Female
Gene
gene expression
mice
pregnancy
RNA
Serum Albumin
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/excr.1996.0108
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30416
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:54
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Scientific Publication
Dual regulation of βlactoglobulin/human serum albumin gene expression by the extracellular matrix in mammary cells from transgenic mice
224
Ilan, N., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Barash, I., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Faerman, A., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, Volcani Center, ARO, Bet Dagan 50250, Israel
Dual regulation of βlactoglobulin/human serum albumin gene expression by the extracellular matrix in mammary cells from transgenic mice
Mammary explants and epithelial cell cultures from transgenic mice carrying the human serum albumin (HSA) gene or minigenes behind the regulatory sequences of the ovine β-lactoglobulin gene were analyzed. Previously we demonstrated that mammary explants from virgin female transgenic mice synthesize and secrete high levels of HSA during the first day in culture. Here we present a detailed analysis of endogenous and transgene expression during the first 20 h of mammary explant cultures. We show that HSA genes as well as endogenous milk protein genes are rapidly induced upon explantation. Unexpectedly, HSA was synthesized also in mammary explants from strains that do not secrete HSA into the milk, indicating the existence of a cryptic potential to express the transgene. Histological examination revealed that some luminal epithelial cells detached from the underlying extracellular matrix (ECM) soon after explantation. Epithelial cell cultures from nonsecreting strains grown on plastic rapidly induced transgene expression and secreted higher levels of HSA into the medium compared to cells grown on collagen. These results suggest that tissue organization and most likely the interaction of epithelial cells with the ECM are intimately involved in the control of HSA transgene expression. © 1998 Academic Press, Inc.
Scientific Publication
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