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פותח על ידי קלירמאש פתרונות בע"מ -
Directional freezing of equine semen in large volumes
Year:
2007
Source of publication :
Reproduction in Domestic Animals
Authors :
גסיטוע, חיים
;
.
Volume :
42
Co-Authors:
Saragusty, J., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gacitua, H., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Pettit, M.T., Cogent Breeding Ltd., Woodhouse Farm, Aldford, Chester, United Kingdom
Arav, A., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
610
To page:
615
(
Total pages:
6
)
Abstract:
Despite its potential impact on the horse industry, sperm cryopreservation is not an established technology throughout the industry, for a number of reasons that include a reduction in pregnancy rate and increased cost per pregnancy. We have evaluated a novel directional freezing technique, based on a multi-thermal gradient (MTG), by comparing it with the conventional, controlled-rate cryopreservation method (CRCM). Ninety-seven ejaculates with ≥50% motility, collected from 31 stallions were each divided into two parts and subsequently frozen by either MTG or CRCM. Frozen samples were then stored in liquid nitrogen until thawing. The two treatments were evaluated by three methods: progressive linear motility (PLM), viability stain and hypoosmotic swelling (HOS) test. High correlation was found between the three evaluation methods for all post-thaw samples. Eighty-eight per cent of the ejaculates frozen by MTG had post-thaw PLM ≥35%, whereas only 59% of the ejaculates frozen by CRCM had such motility. Post-thaw evaluations of samples frozen by MTG and CRCM were: PLM - 50.2 ± 1.5% and 37.4 ± 1.5%, respectively; viability - 53.6 ± 1.5% and 39.5 ± 1.4%, respectively; membrane integrity, as evaluated by HOS - 36.2 ± 1.3% and 26.5 ± 1.1%, respectively. The differences according to all the evaluation methods were highly significant (p < 0.001), and the results indicate that freezing stallion semen by MTG is superior to CRCM. © 2007 The Authors.
Note:
Related Files :
Animal
animal disease
Animals
artificial insemination
Female
horse
Male
pregnancy
Random Allocation
Semen Preservation
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1439-0531.2006.00831.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30482
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:54
Scientific Publication
Directional freezing of equine semen in large volumes
42
Saragusty, J., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gacitua, H., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Pettit, M.T., Cogent Breeding Ltd., Woodhouse Farm, Aldford, Chester, United Kingdom
Arav, A., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Directional freezing of equine semen in large volumes
Despite its potential impact on the horse industry, sperm cryopreservation is not an established technology throughout the industry, for a number of reasons that include a reduction in pregnancy rate and increased cost per pregnancy. We have evaluated a novel directional freezing technique, based on a multi-thermal gradient (MTG), by comparing it with the conventional, controlled-rate cryopreservation method (CRCM). Ninety-seven ejaculates with ≥50% motility, collected from 31 stallions were each divided into two parts and subsequently frozen by either MTG or CRCM. Frozen samples were then stored in liquid nitrogen until thawing. The two treatments were evaluated by three methods: progressive linear motility (PLM), viability stain and hypoosmotic swelling (HOS) test. High correlation was found between the three evaluation methods for all post-thaw samples. Eighty-eight per cent of the ejaculates frozen by MTG had post-thaw PLM ≥35%, whereas only 59% of the ejaculates frozen by CRCM had such motility. Post-thaw evaluations of samples frozen by MTG and CRCM were: PLM - 50.2 ± 1.5% and 37.4 ± 1.5%, respectively; viability - 53.6 ± 1.5% and 39.5 ± 1.4%, respectively; membrane integrity, as evaluated by HOS - 36.2 ± 1.3% and 26.5 ± 1.1%, respectively. The differences according to all the evaluation methods were highly significant (p < 0.001), and the results indicate that freezing stallion semen by MTG is superior to CRCM. © 2007 The Authors.
Scientific Publication
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