The tryptic and chymotryptic fragments of the β-subunit of guanine nucleotide binding proteins in brain are identical to those of retinal transducin

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The tryptic and chymotryptic fragments of the β-subunit of guanine nucleotide binding proteins in brain are identical to those of retinal transducin

Year: 

1985

Source of publication :

FEBS Letters

Authors :

פינס, מרק

.

Volume :

182

Co-Authors: 

Pines, M., Section on Molecular Pathophysiology, Metabolic Diseases Branch, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20205, United States
Gierschik, P., Section on Molecular Pathophysiology, Metabolic Diseases Branch, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20205, United States
Spiegel, A., Section on Molecular Pathophysiology, Metabolic Diseases Branch, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20205, United States

From page: 

355

To page: 

359

(

Total pages: 

5

)

Link :

The tryptic and chymotryptic fragments of the β-subunit of guanine nucleotide binding proteins in brain are identical to those of retinal transducin

Abstract: 

The 35-kDa β-subunit of transducin purified from rod outer segment membranes is cleaved into 2 major fragments by trypsin, and 7 major fragments by chymotrypsin. Identical fragments are visualized by immunoblotting with transducin-β specific antisera after proteolysis of rod outer segment membranes, purified brain guanine nucleotide binding proteins, and brain membranes. The results indicate that the β-subunits of transducin and of brain guanine nucleotide binding proteins are not only similar structurally, but are also similarly oriented in membranes with respect to accessibility to proteolytic enzymes. © 1985.