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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Biological and molecular characterization of tomato spotted wilt virus in Israel
Year:
1997
Source of publication :
Phytoparasitica
Authors :
אנטיגנוס, יחזקאל
;
.
גרה, עבדאללה
;
.
לכמן, עודד
;
.
לפידות, משה
;
.
פרלסמן, מלניה
;
.
רקח, בנימין
;
.
Volume :
25
Co-Authors:
Antignus, Y.
Lapidot, M.
Ganaim, N.
Cohen, J.
Lachman, O.
Pearlsman, M.
Raccah, B.
Gera, A., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
319
To page:
330
(
Total pages:
12
)
Abstract:
Symptoms resembling tomato spotted wilt virus (TSWV) infections were documented among ornamental and vegetable crops in commercial greenhouses and open fields in Israel. Plants exhibiting these symptoms were collected from January 1992 to December 1996. Among cultivated plants analyzed for TSWV by enzyme-linked immunosorbent assay (ELISA), 19 species representing five families were found to be infected; natural infection was also recorded in six plant species of weeds. Virus identity was characterized by host range, serology and electron microscopy. Serological reaction with the isolates, found in Israel, using antisera from different sources as well as the sequence analysis of the nucleocapsid gene, demonstrated that the Israeli isolates of TSWV are a member of tospovirus serogroup I, type I (BR-01 strain). No virus transmission was found in seeds collected from virus-infected vegetable and ornamental crops. A non-radioactive molecular probe derived from the cloned nucleocapsid isolate enables specific detection of the virus in crude sap from infected plants. The detection of TSWV in Israel constitutes a severe potential threat to the ornamental and vegetable industry.
Note:
Related Files :
biology
ELISA
host range
Riboprobe
Seed transmission
Sequence
serology
Tomato spored wilt virus
Tospovirus
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30514
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
Biological and molecular characterization of tomato spotted wilt virus in Israel
25
Antignus, Y.
Lapidot, M.
Ganaim, N.
Cohen, J.
Lachman, O.
Pearlsman, M.
Raccah, B.
Gera, A., Dept. of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Biological and molecular characterization of tomato spotted wilt virus in Israel
Symptoms resembling tomato spotted wilt virus (TSWV) infections were documented among ornamental and vegetable crops in commercial greenhouses and open fields in Israel. Plants exhibiting these symptoms were collected from January 1992 to December 1996. Among cultivated plants analyzed for TSWV by enzyme-linked immunosorbent assay (ELISA), 19 species representing five families were found to be infected; natural infection was also recorded in six plant species of weeds. Virus identity was characterized by host range, serology and electron microscopy. Serological reaction with the isolates, found in Israel, using antisera from different sources as well as the sequence analysis of the nucleocapsid gene, demonstrated that the Israeli isolates of TSWV are a member of tospovirus serogroup I, type I (BR-01 strain). No virus transmission was found in seeds collected from virus-infected vegetable and ornamental crops. A non-radioactive molecular probe derived from the cloned nucleocapsid isolate enables specific detection of the virus in crude sap from infected plants. The detection of TSWV in Israel constitutes a severe potential threat to the ornamental and vegetable industry.
Scientific Publication
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