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פותח על ידי קלירמאש פתרונות בע"מ -
Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase
Year:
2005
Authors :
אשדת, יובל
;
.
Volume :
1724
Co-Authors:
Herbette, S., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Le Menn, A., IUT Génie Biologique, Université d'Avignon, 84029 Avignon, France, UR GAFL, INRA, 84143 Montfavet, France
Rousselle, P., UR GAFL, INRA, 84143 Montfavet, France
Ameglio, T., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Faltin, Z., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, Volcani Center, 50250 Bet-Dagan, Israel
Branlard, G., UMR 1095-ASP, INRA, Université Blaise Pascal, 63039 Clermont-Ferrand, France
Eshdat, Y., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, Volcani Center, 50250 Bet-Dagan, Israel
Julien, J.-L., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Drevet, J.R., UMR 6547-GEEM, CNRS, Université Blaise Pascal, 63177 Aubière, France
Roeckel-Drevet, P., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Facilitators :
From page:
108
To page:
118
(
Total pages:
11
)
Abstract:
To investigate the function of glutathione peroxidase (GPX) in plants, we produced transgenic tomato plants overexpressing an eukaryotic selenium-independent GPX (GPX5). We show here that total GPX activity was increased by 50% in transgenic plants, when compared to control plants transformed with the binary vector without the insert (PZP111). A preliminary two-dimensional electrophoretic protein analysis of the GPX overexpressing plants showed notably a decrease in the accumulation of proteins identified as rubisco small subunit 1 and fructose-1,6-bisphosphate aldolase, two proteins involved in photosynthesis. These observations, together with the fact that in standard culture conditions, GPX-overexpressing plants were not phenotypically distinct from control plants prompted us to challenge the plants with a chilling treatment that is known to affect photosynthesis activity. We found that upon chilling treatment with low light level, photosynthesis was not affected in GPX-overexpressing plants while it was in control plants, as revealed by chlorophyll fluorescence parameters and fructose-1,6-biphosphatase activity. These results suggest that overexpression of a selenium-independent GPX in tomato plants modifies specifically gene expression and leads to modifications of photosynthetic regulation processes. © 2005 Elsevier B.V. All rights reserved.
Note:
Related Files :
Animals
antioxidants
gene overexpression
Glutathione peroxidase
mice
phenotype
photosynthesis
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.bbagen.2005.04.018
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30518
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase
1724
Herbette, S., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Le Menn, A., IUT Génie Biologique, Université d'Avignon, 84029 Avignon, France, UR GAFL, INRA, 84143 Montfavet, France
Rousselle, P., UR GAFL, INRA, 84143 Montfavet, France
Ameglio, T., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Faltin, Z., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, Volcani Center, 50250 Bet-Dagan, Israel
Branlard, G., UMR 1095-ASP, INRA, Université Blaise Pascal, 63039 Clermont-Ferrand, France
Eshdat, Y., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, Volcani Center, 50250 Bet-Dagan, Israel
Julien, J.-L., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Drevet, J.R., UMR 6547-GEEM, CNRS, Université Blaise Pascal, 63177 Aubière, France
Roeckel-Drevet, P., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase
To investigate the function of glutathione peroxidase (GPX) in plants, we produced transgenic tomato plants overexpressing an eukaryotic selenium-independent GPX (GPX5). We show here that total GPX activity was increased by 50% in transgenic plants, when compared to control plants transformed with the binary vector without the insert (PZP111). A preliminary two-dimensional electrophoretic protein analysis of the GPX overexpressing plants showed notably a decrease in the accumulation of proteins identified as rubisco small subunit 1 and fructose-1,6-bisphosphate aldolase, two proteins involved in photosynthesis. These observations, together with the fact that in standard culture conditions, GPX-overexpressing plants were not phenotypically distinct from control plants prompted us to challenge the plants with a chilling treatment that is known to affect photosynthesis activity. We found that upon chilling treatment with low light level, photosynthesis was not affected in GPX-overexpressing plants while it was in control plants, as revealed by chlorophyll fluorescence parameters and fructose-1,6-biphosphatase activity. These results suggest that overexpression of a selenium-independent GPX in tomato plants modifies specifically gene expression and leads to modifications of photosynthetic regulation processes. © 2005 Elsevier B.V. All rights reserved.
Scientific Publication
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