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פותח על ידי קלירמאש פתרונות בע"מ -
Vegetative micro-cloning to sustain biodiversity of threatened Moringa species
Year:
2009
Authors :
גאבה, ויקטור
;
.
גפן, טניה
;
.
ואקנין, יפתח
;
.
טביב, יונה
;
.
שטייניץ, בנימין
;
.
Volume :
45
Co-Authors:
Steinitz, B., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Tabib, Y., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Gaba, V., Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Gefen, T., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Vaknin, Y., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Facilitators :
From page:
65
To page:
71
(
Total pages:
7
)
Abstract:
Efficient vegetative cloning in vitro requires definition of plant growth regulator regimes for each genotype, and therefore formulation of a uniform culture protocol for a genetically heterogeneous wild or uncultivated plant population is often impossible. The likelihood of cloning a wide array of plant genotypes by avoiding the use of plant growth regulator(s) was explored with Moringa oleifera Lamk., Moringa stenopetala (Baker f.) Cufod, and Moringa peregrina Forssk. ex Fiori tree seedlings. Propagation was achieved by multiple shoot regeneration from the cotyledonary node of decapitated seedlings, followed by axillary shoot growth from single node shoot segments and rooting of excised shoots. All steps were accomplished on basal Murashige and Skoog medium without plant growth regulator supplements. The results revealed competence for generation of multiple shoots from cotyledonary node tissue, stimulated by repeated shoot harvest, in seedlings of all three tree species. Tens of plants per seedling were regenerated in about 4 mo from culture initiation. In a given species clone size was seedling-dependent, which presumably stems from genotypic variability among seedlings in regeneration ability in vitro. By this means the laborious search for a plant growth regulator regime suitable for organogenesis induction and adapted per genotype became redundant, and biodiversity of the seed germplasm could be maintained. The approach ideally suits establishment of clones of wild plants of endangered species, like those of the Moringaceae, species with high ability for producing supplementary shoots, and without the need to add plant growth regulators, including the rooting stage. © 2008 The Society for In Vitro Biology.
Note:
Related Files :
biodiversity
cloning
Conservation
Endangered plant
Moringa
Moringaceae
Moringa oleifera
Moringa peregrina
Moringa stenopetala
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s11627-008-9162-x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30549
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
You may also be interested in
Scientific Publication
Vegetative micro-cloning to sustain biodiversity of threatened Moringa species
45
Steinitz, B., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Tabib, Y., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Gaba, V., Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Gefen, T., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Vaknin, Y., Department of Plant Genetics and Vegetable Research, Institute of Plant Sciences, Volcani Center, P.O.B 6, Bet Dagan 50250, Israel
Vegetative micro-cloning to sustain biodiversity of threatened Moringa species
Efficient vegetative cloning in vitro requires definition of plant growth regulator regimes for each genotype, and therefore formulation of a uniform culture protocol for a genetically heterogeneous wild or uncultivated plant population is often impossible. The likelihood of cloning a wide array of plant genotypes by avoiding the use of plant growth regulator(s) was explored with Moringa oleifera Lamk., Moringa stenopetala (Baker f.) Cufod, and Moringa peregrina Forssk. ex Fiori tree seedlings. Propagation was achieved by multiple shoot regeneration from the cotyledonary node of decapitated seedlings, followed by axillary shoot growth from single node shoot segments and rooting of excised shoots. All steps were accomplished on basal Murashige and Skoog medium without plant growth regulator supplements. The results revealed competence for generation of multiple shoots from cotyledonary node tissue, stimulated by repeated shoot harvest, in seedlings of all three tree species. Tens of plants per seedling were regenerated in about 4 mo from culture initiation. In a given species clone size was seedling-dependent, which presumably stems from genotypic variability among seedlings in regeneration ability in vitro. By this means the laborious search for a plant growth regulator regime suitable for organogenesis induction and adapted per genotype became redundant, and biodiversity of the seed germplasm could be maintained. The approach ideally suits establishment of clones of wild plants of endangered species, like those of the Moringaceae, species with high ability for producing supplementary shoots, and without the need to add plant growth regulators, including the rooting stage. © 2008 The Society for In Vitro Biology.
Scientific Publication
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