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פותח על ידי קלירמאש פתרונות בע"מ -
An adhesion-defective mutant of ruminococcus albus sy3 is impaired in its capability to degrade cellulose
Year:
1998
Source of publication :
Journal of Applied Microbiology
Authors :
בן גדליה, דניאל
;
.
מירון, יהושע
;
.
Volume :
84
Co-Authors:
Miron, J., Metabolic Unit, Volcani Center, ARO, Bet Dagan, Israel, Metabolic Unit, Agricultural Research Organization, Volcani Center, P.O.Box 6, Bet Dagan, 50250, Israel
Morag, E., Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Bayer, E.A., Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Lamed, R., Center for Biotechnology, George S. Wise Fac. of Life Science, Tel Aviv University, Ramat Aviv, Israel
Ben-Ghedalia, D., Metabolic Unit, Volcani Center, ARO, Bet Dagan, Israel
Facilitators :
From page:
249
To page:
254
(
Total pages:
6
)
Abstract:
An adhesion defective mutant of Ruminococcus albus SY3 was isolated by a subtractive enrichment procedure, which involved repetitive adsorption of cellobiose-grown cells to cellulose. The growth characteristics of the mutant were compared with those of the wild type. Like the wild-type cells, the mutant was capable of growing on soluble substrates, i.e. cellobiose and xylan. However, in contrast to the wild type strain, the mutant was impaired in its capacity to utilize insoluble substrates, e.g. crystalline cellulose, acid-swollen cellulose or alfalfa cell walls. Scanning electron microscopy revealed protuberance-like surface structures on the wild-type strain which were absent on the mutant. The levels of endoglucanase and xylanase enzymatic activities released into the extracellular culture fluid were higher in the wild type compared to the mutant. However, Avicelase activity was not detected in the extracellular culture fluid of either strains when grown on cellobiose.
Note:
Related Files :
Adsorption
alfalfa
cellobiose
cellulase
Cellulose
Degradation
enzyme activity
Ruminococcus
xylan endo 1,3 beta xylosidase
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1365-2672.1998.00336.x
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30583
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
An adhesion-defective mutant of ruminococcus albus sy3 is impaired in its capability to degrade cellulose
84
Miron, J., Metabolic Unit, Volcani Center, ARO, Bet Dagan, Israel, Metabolic Unit, Agricultural Research Organization, Volcani Center, P.O.Box 6, Bet Dagan, 50250, Israel
Morag, E., Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Bayer, E.A., Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Lamed, R., Center for Biotechnology, George S. Wise Fac. of Life Science, Tel Aviv University, Ramat Aviv, Israel
Ben-Ghedalia, D., Metabolic Unit, Volcani Center, ARO, Bet Dagan, Israel
An adhesion-defective mutant of ruminococcus albus sy3 is impaired in its capability to degrade cellulose
An adhesion defective mutant of Ruminococcus albus SY3 was isolated by a subtractive enrichment procedure, which involved repetitive adsorption of cellobiose-grown cells to cellulose. The growth characteristics of the mutant were compared with those of the wild type. Like the wild-type cells, the mutant was capable of growing on soluble substrates, i.e. cellobiose and xylan. However, in contrast to the wild type strain, the mutant was impaired in its capacity to utilize insoluble substrates, e.g. crystalline cellulose, acid-swollen cellulose or alfalfa cell walls. Scanning electron microscopy revealed protuberance-like surface structures on the wild-type strain which were absent on the mutant. The levels of endoglucanase and xylanase enzymatic activities released into the extracellular culture fluid were higher in the wild type compared to the mutant. However, Avicelase activity was not detected in the extracellular culture fluid of either strains when grown on cellobiose.
Scientific Publication
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