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פותח על ידי קלירמאש פתרונות בע"מ -
The hydrolysis of lucerne cell‐wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria
Year:
1991
Source of publication :
Journal of Applied Bacteriology
Authors :
מירון, יהושע
;
.
Volume :
70
Co-Authors:
Miron, J., Metabolic Unit, Institute of Animal Science, The Volcani Center, ARO, Bet Dagan, Israel
Facilitators :
From page:
245
To page:
252
(
Total pages:
8
)
Abstract:
The defined ruminal bacterial strains Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD1, Ruminococcus albus 7, Butyrivibrio fibrisolvens D1, and Bacteroides ruminicola GA33 were grown, in monocultures or as combinations of pair strains, on isolated lucerne cell‐walls (CW) as the sole carbohydrate substrate. Fibrobacter succinogenes S85 was the dominant strain determining extent of CW hydrolysis in all combinations with S85. The hydrolysis of cellulose, xylan, hemicellulose side‐sugars, and total CW monosaccharides by pure S85 were: 58·8, 47·3, 66·9 and 57·0%, respectively. The strains combination S85 plus D1 comprised the highest complementary effect, increasing significantly the hydrolysis of cellulose and total CW monosaccharides by 16% and 13%, respectively, above the values obtained by pure S85. This complementation was expressed also in growth pattern of bacteria. The monocultures of FD1, D1 and GA33 had very little hydrolytic effect on lucerne cellulose, but higher effects on xylan and hemicellulose side‐sugars. The combinations D1 plus GA33 and 7 plus GA33 were complementary in the hydrolysis of all CW polysaccharides. The combinations FD1 plus D1, FD1 plus GA33, and 7 plus D1 were complementary only with respect to hemicellulose hydrolysis. On the other hand, the cellulolytic combinations S85 plus FD1, S85 plus 7 and FD1 plus 7 demonstrated negative interactions in lucerne CW polysaccharides hydrolysis. Under scanning electron microscopy (SEM), S85 comprised the most dense layer of bacterial cell mass attached to and colonized on CW particles. The cell surface topology of the cellulolytic strains S85, FD1 and 7 attached to CW particles was specified by a coat of characteristic protuberant structures. Copyright © 1991, Wiley Blackwell. All rights reserved
Note:
Related Files :
Animal
animal cell
carbohydrates
electron microscopy
Hydrolysis
Medicago sativa
monosaccharide
Rumen
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-2672.1991.tb02932.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30663
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Scientific Publication
The hydrolysis of lucerne cell‐wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria
70
Miron, J., Metabolic Unit, Institute of Animal Science, The Volcani Center, ARO, Bet Dagan, Israel
The hydrolysis of lucerne cell‐wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria
The defined ruminal bacterial strains Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD1, Ruminococcus albus 7, Butyrivibrio fibrisolvens D1, and Bacteroides ruminicola GA33 were grown, in monocultures or as combinations of pair strains, on isolated lucerne cell‐walls (CW) as the sole carbohydrate substrate. Fibrobacter succinogenes S85 was the dominant strain determining extent of CW hydrolysis in all combinations with S85. The hydrolysis of cellulose, xylan, hemicellulose side‐sugars, and total CW monosaccharides by pure S85 were: 58·8, 47·3, 66·9 and 57·0%, respectively. The strains combination S85 plus D1 comprised the highest complementary effect, increasing significantly the hydrolysis of cellulose and total CW monosaccharides by 16% and 13%, respectively, above the values obtained by pure S85. This complementation was expressed also in growth pattern of bacteria. The monocultures of FD1, D1 and GA33 had very little hydrolytic effect on lucerne cellulose, but higher effects on xylan and hemicellulose side‐sugars. The combinations D1 plus GA33 and 7 plus GA33 were complementary in the hydrolysis of all CW polysaccharides. The combinations FD1 plus D1, FD1 plus GA33, and 7 plus D1 were complementary only with respect to hemicellulose hydrolysis. On the other hand, the cellulolytic combinations S85 plus FD1, S85 plus 7 and FD1 plus 7 demonstrated negative interactions in lucerne CW polysaccharides hydrolysis. Under scanning electron microscopy (SEM), S85 comprised the most dense layer of bacterial cell mass attached to and colonized on CW particles. The cell surface topology of the cellulolytic strains S85, FD1 and 7 attached to CW particles was specified by a coat of characteristic protuberant structures. Copyright © 1991, Wiley Blackwell. All rights reserved
Scientific Publication
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