Norelli, J.L., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States Bassett, C., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States Artlip, T., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States Gidoni, D., ARO - The Volcani Center, Bet Dagan, Israel Flaishman, M., ARO - The Volcani Center, Bet Dagan, Israel Aldwinckle, H.S., Cornell University, Department of Plant Pathology, Geneva, NY, United States Malnoy, M., Cornell University, Department of Plant Pathology, Geneva, NY, United States Borejsza-Wysocka, E.E., Cornell University, Department of Plant Pathology, Geneva, NY, United States
A chemically inducible and a light inducible DNA promoter are being developed and evaluated for their ability to regulate gene expression in transgenic apple. The estradiol-induced XVE gene expression system was cloned into a binary vector (pBinPlusARS) compatible for use in Agrobacterium-mediated transformation of apple. To evaluate the estradiol-inducible binary vectors, a GUS marker gene was cloned into pBinPlusARS.XVE and pBinPlusARS.XVE.Gateway. When evaluated in tobacco under non-inducing conditions, GUS expression from the GUS containing XVE constructs was indistinguishable from that of an empty vector (no GUS coding region) negative control. However, in the presence of estradiol, GUS expression from the GUS containing XVE constructs was greater than that from a 35S promoter positive control. To evaluate the activity of the light inducible promoter of the peach chlorophyll a/b binding (CAB) protein and XVE in transgenic apple, binary vectors were constructed to allow comparison of test promoter activity with 35S activity.
Norelli, J.L., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States Bassett, C., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States Artlip, T., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States Gidoni, D., ARO - The Volcani Center, Bet Dagan, Israel Flaishman, M., ARO - The Volcani Center, Bet Dagan, Israel Aldwinckle, H.S., Cornell University, Department of Plant Pathology, Geneva, NY, United States Malnoy, M., Cornell University, Department of Plant Pathology, Geneva, NY, United States Borejsza-Wysocka, E.E., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Inducible DNA promoters for use in apple
A chemically inducible and a light inducible DNA promoter are being developed and evaluated for their ability to regulate gene expression in transgenic apple. The estradiol-induced XVE gene expression system was cloned into a binary vector (pBinPlusARS) compatible for use in Agrobacterium-mediated transformation of apple. To evaluate the estradiol-inducible binary vectors, a GUS marker gene was cloned into pBinPlusARS.XVE and pBinPlusARS.XVE.Gateway. When evaluated in tobacco under non-inducing conditions, GUS expression from the GUS containing XVE constructs was indistinguishable from that of an empty vector (no GUS coding region) negative control. However, in the presence of estradiol, GUS expression from the GUS containing XVE constructs was greater than that from a 35S promoter positive control. To evaluate the activity of the light inducible promoter of the peach chlorophyll a/b binding (CAB) protein and XVE in transgenic apple, binary vectors were constructed to allow comparison of test promoter activity with 35S activity.