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פותח על ידי קלירמאש פתרונות בע"מ -
Inducible DNA promoters for use in apple
Year:
2007
Source of publication :
Acta Horticulturae
Authors :
גדעוני, דוד
;
.
פליישמן, משה
;
.
Volume :
738
Co-Authors:
Norelli, J.L., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States
Bassett, C., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States
Artlip, T., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States
Gidoni, D., ARO - The Volcani Center, Bet Dagan, Israel
Flaishman, M., ARO - The Volcani Center, Bet Dagan, Israel
Aldwinckle, H.S., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Malnoy, M., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Borejsza-Wysocka, E.E., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Facilitators :
From page:
329
To page:
334
(
Total pages:
6
)
Abstract:
A chemically inducible and a light inducible DNA promoter are being developed and evaluated for their ability to regulate gene expression in transgenic apple. The estradiol-induced XVE gene expression system was cloned into a binary vector (pBinPlusARS) compatible for use in Agrobacterium-mediated transformation of apple. To evaluate the estradiol-inducible binary vectors, a GUS marker gene was cloned into pBinPlusARS.XVE and pBinPlusARS.XVE.Gateway. When evaluated in tobacco under non-inducing conditions, GUS expression from the GUS containing XVE constructs was indistinguishable from that of an empty vector (no GUS coding region) negative control. However, in the presence of estradiol, GUS expression from the GUS containing XVE constructs was greater than that from a 35S promoter positive control. To evaluate the activity of the light inducible promoter of the peach chlorophyll a/b binding (CAB) protein and XVE in transgenic apple, binary vectors were constructed to allow comparison of test promoter activity with 35S activity.
Note:
Related Files :
Agrobacterium
CAB
Gateway
Malus x domestica
Nicotiana tabacum
Prunus persica
Rhizobium
Transgenic
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
30807
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
Scientific Publication
Inducible DNA promoters for use in apple
738
Norelli, J.L., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States
Bassett, C., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States
Artlip, T., USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV, United States
Gidoni, D., ARO - The Volcani Center, Bet Dagan, Israel
Flaishman, M., ARO - The Volcani Center, Bet Dagan, Israel
Aldwinckle, H.S., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Malnoy, M., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Borejsza-Wysocka, E.E., Cornell University, Department of Plant Pathology, Geneva, NY, United States
Inducible DNA promoters for use in apple
A chemically inducible and a light inducible DNA promoter are being developed and evaluated for their ability to regulate gene expression in transgenic apple. The estradiol-induced XVE gene expression system was cloned into a binary vector (pBinPlusARS) compatible for use in Agrobacterium-mediated transformation of apple. To evaluate the estradiol-inducible binary vectors, a GUS marker gene was cloned into pBinPlusARS.XVE and pBinPlusARS.XVE.Gateway. When evaluated in tobacco under non-inducing conditions, GUS expression from the GUS containing XVE constructs was indistinguishable from that of an empty vector (no GUS coding region) negative control. However, in the presence of estradiol, GUS expression from the GUS containing XVE constructs was greater than that from a 35S promoter positive control. To evaluate the activity of the light inducible promoter of the peach chlorophyll a/b binding (CAB) protein and XVE in transgenic apple, binary vectors were constructed to allow comparison of test promoter activity with 35S activity.
Scientific Publication
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