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פותח על ידי קלירמאש פתרונות בע"מ -
Citrus tristeza virus replicates and forms infectious virions in protoplasts of resistant citrus relatives
Year:
2004
Source of publication :
Molecular Breeding
Authors :
מוואסי, מוניר
;
.
Volume :
14
Co-Authors:
Albiach-Marti, M.R., Inst. Valenciano de Invest. Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain, Spain
Grosser, J.W., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Gowda, S., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Mawassi, M., Virology Department, Volcani Center, Bet-Dagan, 50250, Israel, Israel
Satyanarayana, T., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Garnsey, S.M., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Dawson, W.O., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Facilitators :
From page:
117
To page:
128
(
Total pages:
12
)
Abstract:
Citrus tristeza virus (CTV) is the most economically important viral disease of citrus worldwide. Cultivars with improved CTV tolerance or resistance are needed to manage CTV-induced diseases. The citrus relatives Poncirus trifoliata (L.) Raf., Swinglea glutinosa (Blanco) Merr., and Severinia buxifolia (Poir) Ten. are potential sources of CTV resistance, but their resistance mechanisms are poorly characterized. As a first step to examine the mechanisms of resistance to CTV in these citrus relatives and selected Citrus × Poncirus hybrids, it was necessary to develop methods for protoplast isolation and viral inoculation to allow examination of CTV multiplication in this range of citrus varieties and relatives. Leaf and/or cultured cell protoplasts were isolated and inoculated with four biologically distinct CTV isolates. Northern-blot hybridization analyses for progeny RNAs and immuno-electron microscopy assays for newly produced virions showed that CTV replicated and produced infectious particles in protoplasts from all of the resistant plants tested. These results suggest that resistance to CTV observed at the plant level results from a lack of virus movement and/or some induced resistance response, rather than lack of viral multiplication at the cellular level.
Note:
Related Files :
Citrus tristeza virus
CTV resistance
Poncirus trifoliata
Protoplasts
Severinia
Swinglea
virus replication
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/B:MOLB.0000038000.51218.a7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30836
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
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Scientific Publication
Citrus tristeza virus replicates and forms infectious virions in protoplasts of resistant citrus relatives
14
Albiach-Marti, M.R., Inst. Valenciano de Invest. Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain, Spain
Grosser, J.W., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Gowda, S., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Mawassi, M., Virology Department, Volcani Center, Bet-Dagan, 50250, Israel, Israel
Satyanarayana, T., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Garnsey, S.M., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Dawson, W.O., Citrus Research and Education Center, University of Florida, 700 Exp. Stn. Rd., Lake Alfred, F., United States
Citrus tristeza virus replicates and forms infectious virions in protoplasts of resistant citrus relatives
Citrus tristeza virus (CTV) is the most economically important viral disease of citrus worldwide. Cultivars with improved CTV tolerance or resistance are needed to manage CTV-induced diseases. The citrus relatives Poncirus trifoliata (L.) Raf., Swinglea glutinosa (Blanco) Merr., and Severinia buxifolia (Poir) Ten. are potential sources of CTV resistance, but their resistance mechanisms are poorly characterized. As a first step to examine the mechanisms of resistance to CTV in these citrus relatives and selected Citrus × Poncirus hybrids, it was necessary to develop methods for protoplast isolation and viral inoculation to allow examination of CTV multiplication in this range of citrus varieties and relatives. Leaf and/or cultured cell protoplasts were isolated and inoculated with four biologically distinct CTV isolates. Northern-blot hybridization analyses for progeny RNAs and immuno-electron microscopy assays for newly produced virions showed that CTV replicated and produced infectious particles in protoplasts from all of the resistant plants tested. These results suggest that resistance to CTV observed at the plant level results from a lack of virus movement and/or some induced resistance response, rather than lack of viral multiplication at the cellular level.
Scientific Publication
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