נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species
Year:
2017
Source of publication :
Journal of Experimental Botany
Authors :
אגבאריה, איימן
;
.
ברנדסמה, דניה
;
.
חמאיסי, בלאל
;
.
לוגסי, ניצן
;
.
פידל, לנה
;
.
קוטפאלי, ג'איארם
;
.
Volume :
68
Co-Authors:
Kelly, G., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Lugassi, N., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Wolf, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Khamaisi, B., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Brandsma, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel, Horticulture and Product Physiology, Wageningen University, PO Box 630, Wageningen, Netherlands
Kottapalli, J., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Fidel, L., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Ben-Zvi, B., Faculty of Life Sciences, Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Israel
Egbaria, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Acheampong, A.K., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Zheng, C., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Or, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Distelfeld, A., Faculty of Life Sciences, Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Israel
David-Schwartz, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Carmi, N., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Facilitators :
From page:
2885
To page:
2897
(
Total pages:
13
)
Abstract:
To date, guard cell promoters have been examined in only a few species, primarily annual dicots. A partial segment of the potato (Solanum tuberosum) KST1 promoter (KST1 partial promoter, KST1 ppro) has previously been shown to confer guard cell expression in potato, tomato (Solanum lycopersicum), citrus [Troyer citrange (C. sinensis×Poncirus trifoliata)], and Arabidopsis (Arabidopsis thaliana). Here, we describe an extensive analysis of the expression pattern of KST1 ppro in eight (previously reported, as well as new) species from five different angiosperm families, including the Solanaceae and the Cucurbitaceae, Arabidopsis, the monocot barley (Hordeum vulgare), and two perennial species: grapevine (Vitis vinifera) and citrus. Using confocal imaging and three-dimensional movies, we demonstrate that KST1 ppro drives guard cell expression in all of these species, making it the first dicot-originated guard cell promoter shown to be active in a monocot and the first promoter reported to confer guard cell expression in barley and cucumber (Cucumis sativus). The results presented here indicate that KST1 ppro can be used to drive constitutive guard cell expression in monocots and dicots and in both annual and perennial plants. In addition, we show that the KST1 ppro is active in guard cells shortly after the symmetric division of the guard mother cell and generates stable expression in mature guard cells. This allows us to follow the spatial and temporal distribution of stomata in cotyledons and true leaves. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Note:
Related Files :
Confocal imaging
GFP
guard cell-specific promoters
KST1 promoter
stomata
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/erx159
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30876
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
Scientific Publication
The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species
68
Kelly, G., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Lugassi, N., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Wolf, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Khamaisi, B., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Brandsma, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel, Horticulture and Product Physiology, Wageningen University, PO Box 630, Wageningen, Netherlands
Kottapalli, J., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Fidel, L., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Ben-Zvi, B., Faculty of Life Sciences, Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Israel
Egbaria, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Acheampong, A.K., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Zheng, C., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Or, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Distelfeld, A., Faculty of Life Sciences, Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Israel
David-Schwartz, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Carmi, N., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species
To date, guard cell promoters have been examined in only a few species, primarily annual dicots. A partial segment of the potato (Solanum tuberosum) KST1 promoter (KST1 partial promoter, KST1 ppro) has previously been shown to confer guard cell expression in potato, tomato (Solanum lycopersicum), citrus [Troyer citrange (C. sinensis×Poncirus trifoliata)], and Arabidopsis (Arabidopsis thaliana). Here, we describe an extensive analysis of the expression pattern of KST1 ppro in eight (previously reported, as well as new) species from five different angiosperm families, including the Solanaceae and the Cucurbitaceae, Arabidopsis, the monocot barley (Hordeum vulgare), and two perennial species: grapevine (Vitis vinifera) and citrus. Using confocal imaging and three-dimensional movies, we demonstrate that KST1 ppro drives guard cell expression in all of these species, making it the first dicot-originated guard cell promoter shown to be active in a monocot and the first promoter reported to confer guard cell expression in barley and cucumber (Cucumis sativus). The results presented here indicate that KST1 ppro can be used to drive constitutive guard cell expression in monocots and dicots and in both annual and perennial plants. In addition, we show that the KST1 ppro is active in guard cells shortly after the symmetric division of the guard mother cell and generates stable expression in mature guard cells. This allows us to follow the spatial and temporal distribution of stomata in cotyledons and true leaves. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Scientific Publication
You may also be interested in