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The cucurbit pathogenic bacterium acidovorax citrulli requires a polar flagellum for full virulence before and after host-tissue penetration
Year:
2011
Authors :
בהר, אופיר
;
.
Volume :
24
Co-Authors:
Bahar, O., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P. O. Box 12, Rehovot 76100, Israel
Levi, N., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P. O. Box 12, Rehovot 76100, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P. O. Box 12, Rehovot 76100, Israel
Facilitators :
From page:
1040
To page:
1050
(
Total pages:
11
)
Abstract:
Acidovorax citrulli causes seedling blight and bacterial fruit blotch of cucurbits. Previous reports demonstrated the contribution of type IV pili (T4P) to A. citrulli virulence and to systemic infection of melon seedlings. Microfluidic flow-chamber assays demonstrated the involvement of T4P in surface adhesion and biofilm formation, whereas polar flagella did not appear to contribute to either of these features. On the other hand, a transposon mutant impaired in the biosynthesis of polar flagella was identified in screens for reduced virulence of an A. citrulli mutant library. Further characterization of polar flagellum mutants confirmed that A. citrulli requires a polar flagellum for full virulence on melon plants. Foliage and stem inoculation experiments revealed that polar flagella contribute to A. citrulli virulence and growth in planta at both pre- and post-host-tissue penetration. Interestingly, light microscope observations revealed that almost all A. citrulli wild-type cells extracted from the xylem sap of stem-inoculated melon seedlings remained motile, supporting the importance of this organelle in virulence and colonization of the host vascular system. We also report a negative effect of polar flagellum impairment on T4P-mediated twitching motility of A. citrulli and discuss a possible co-regulation of these two motility machineries in this bacterium. © 2011 The American Phytopathological Society.
Note:
Related Files :
Cucumis melo
Genetics
Growth, Development and Aging
Microbiology
mutation
Plant Disease
Plant Diseases
ultrastructure
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/MPMI-02-11-0041
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30878
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:57
You may also be interested in
Scientific Publication
The cucurbit pathogenic bacterium acidovorax citrulli requires a polar flagellum for full virulence before and after host-tissue penetration
24
Bahar, O., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P. O. Box 12, Rehovot 76100, Israel
Levi, N., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P. O. Box 12, Rehovot 76100, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P. O. Box 12, Rehovot 76100, Israel
The cucurbit pathogenic bacterium acidovorax citrulli requires a polar flagellum for full virulence before and after host-tissue penetration
Acidovorax citrulli causes seedling blight and bacterial fruit blotch of cucurbits. Previous reports demonstrated the contribution of type IV pili (T4P) to A. citrulli virulence and to systemic infection of melon seedlings. Microfluidic flow-chamber assays demonstrated the involvement of T4P in surface adhesion and biofilm formation, whereas polar flagella did not appear to contribute to either of these features. On the other hand, a transposon mutant impaired in the biosynthesis of polar flagella was identified in screens for reduced virulence of an A. citrulli mutant library. Further characterization of polar flagellum mutants confirmed that A. citrulli requires a polar flagellum for full virulence on melon plants. Foliage and stem inoculation experiments revealed that polar flagella contribute to A. citrulli virulence and growth in planta at both pre- and post-host-tissue penetration. Interestingly, light microscope observations revealed that almost all A. citrulli wild-type cells extracted from the xylem sap of stem-inoculated melon seedlings remained motile, supporting the importance of this organelle in virulence and colonization of the host vascular system. We also report a negative effect of polar flagellum impairment on T4P-mediated twitching motility of A. citrulli and discuss a possible co-regulation of these two motility machineries in this bacterium. © 2011 The American Phytopathological Society.
Scientific Publication
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