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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Callus induction and regeneration in Spirodela and Lemna
Year:
2004
Source of publication :
Plant Cell Reports
Authors :
וישנבצקי, ג'יין
;
.
פליישמן, משה
;
.
פרל, אבי
;
.
Volume :
22
Co-Authors:
Li, J., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel
Jain, M., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel, Dept. of Molec. Biol. and Microbiol., University of Central Florida, Orlando, FL 32826-3227, United States
Vunsh, R., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel
Vishnevetsky, J., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Hanania, U., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Flaishman, M., Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel
Facilitators :
From page:
457
To page:
464
(
Total pages:
8
)
Abstract:
The development of tissue culture systems in duckweeds has, to date, been limited to species of the genus Lemna. We report here the establishment of an efficient tissue culture cycle (callus induction, callus growth and plant regeneration) for Spirodela oligorrhiza Hegelm SP, Spirodela punctata 8717 and Lemna gibba var. Hurfeish. Significant differences were found among the three duckweed species pertaining to carbohydrate and phytohormone requirements for callus induction, callus growth and frond regeneration. In vitro incubation with poorly assimilated carbohydrates such as galactose (S. oligorrhiza SP and L. gibba var. Hurfeish) and sorbitol (S. punctata 8717) as sole carbon source yielded high levels of callus induction on phytohormone-supplemented medium. Sorbitol is required for optimal callus growth of S. oligorrhiza SP and S. punctata 8717, while sucrose is required for callus growth of L. gibba var. Hurfeish. Sucrose either alone (S. oligorrhiza SP, L. gibba var. Hurfeish) or in addition to sorbitol (S. punctata 8717) is required for frond regeneration.
Note:
Related Files :
Araceae
carbohydrate metabolism
carbohydrates
Galactose
Growth, Development and Aging
hormones
Phytohormones
tissue culture
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00299-003-0724-4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30915
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:58
Scientific Publication
Callus induction and regeneration in Spirodela and Lemna
22
Li, J., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel
Jain, M., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel, Dept. of Molec. Biol. and Microbiol., University of Central Florida, Orlando, FL 32826-3227, United States
Vunsh, R., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel
Vishnevetsky, J., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Hanania, U., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Flaishman, M., Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, 50250 Bet Dagan, Israel
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel
Callus induction and regeneration in Spirodela and Lemna
The development of tissue culture systems in duckweeds has, to date, been limited to species of the genus Lemna. We report here the establishment of an efficient tissue culture cycle (callus induction, callus growth and plant regeneration) for Spirodela oligorrhiza Hegelm SP, Spirodela punctata 8717 and Lemna gibba var. Hurfeish. Significant differences were found among the three duckweed species pertaining to carbohydrate and phytohormone requirements for callus induction, callus growth and frond regeneration. In vitro incubation with poorly assimilated carbohydrates such as galactose (S. oligorrhiza SP and L. gibba var. Hurfeish) and sorbitol (S. punctata 8717) as sole carbon source yielded high levels of callus induction on phytohormone-supplemented medium. Sorbitol is required for optimal callus growth of S. oligorrhiza SP and S. punctata 8717, while sucrose is required for callus growth of L. gibba var. Hurfeish. Sucrose either alone (S. oligorrhiza SP, L. gibba var. Hurfeish) or in addition to sorbitol (S. punctata 8717) is required for frond regeneration.
Scientific Publication
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