נגישות
menu      
חיפוש מתקדם
FEBS Letters
Shahak, Y., Biochemistry Department, The Weizmann Institute of Science, Rehovot, 76100, Israel
Admon, A., Biochemistry Department, The Weizmann Institute of Science, Rehovot, 76100, Israel
Avron, M., Biochemistry Department, The Weizmann Institute of Science, Rehovot, 76100, Israel
Formation of a transmembrane electric potential coupled to ATP hydrolysis is demonstrated in chloroplast ATPase complex containing proteoliposomes. The ATP-induced signals were detected through absorbance changes of the membrane potential-responding dye oxonol VI. They were inhibited by the specific energy-transfer inhibitor, tentoxin and the ionophore valinomycin while stimulated by nigericin. Calibration of the transmembrane potential signal was possible by the application of a proton diffusion potential. The ATP-induced transmembrane potential was estimated to be 40-50 mV. © 1982.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Transmembrane electrical potential formation by chloroplast ATPase complex (CF1CF0) proteoliposomes
150
Shahak, Y., Biochemistry Department, The Weizmann Institute of Science, Rehovot, 76100, Israel
Admon, A., Biochemistry Department, The Weizmann Institute of Science, Rehovot, 76100, Israel
Avron, M., Biochemistry Department, The Weizmann Institute of Science, Rehovot, 76100, Israel
Transmembrane electrical potential formation by chloroplast ATPase complex (CF1CF0) proteoliposomes
Formation of a transmembrane electric potential coupled to ATP hydrolysis is demonstrated in chloroplast ATPase complex containing proteoliposomes. The ATP-induced signals were detected through absorbance changes of the membrane potential-responding dye oxonol VI. They were inhibited by the specific energy-transfer inhibitor, tentoxin and the ionophore valinomycin while stimulated by nigericin. Calibration of the transmembrane potential signal was possible by the application of a proton diffusion potential. The ATP-induced transmembrane potential was estimated to be 40-50 mV. © 1982.
Scientific Publication
You may also be interested in