Co-Authors:
Bischoff, V., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany, Institut Jean-Pierre Bourgin, UMR 1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, 78026 Versailles, France
Nita, S., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Neumetzler, L., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Schindelasch, D., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Urbain, A., Institut Jean-Pierre Bourgin, UMR 1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, 78026 Versailles, France
Eshed, R., Department of Ornamental Horticulture, The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel
Persson, S., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Delmer, D., Section of Plant Biology, University of California, Davis, CA 95616, United States, 33 Riverside Dr., New York, NY 10023, United States
Scheible, W.-R., Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Abstract:
The Arabidopsis (Arabidopsis thaliana) trichome birefringence (tbr) mutant has severely reduced crystalline cellulose in trichomes, but the molecular nature of TBR was unknown. We determined TBR to belong to the plant-specific DUF231 domain gene family comprising 46 members of unknown function in Arabidopsis. The genes harbor another plant-specific domain, called the TBL domain, which contains a conserved GDSL motif known from some esterases/lipases. TBR and TBR-like3 (TBL3) are transcriptionally coordinated with primary and secondary CELLULOSE SYNTHASE (CESA) genes, respectively. The tbr and tbl3 mutants hold lower levels of crystalline cellulose and have altered pectin composition in trichomes and stems, respectively, tissues generally thought to contain mainly secondary wall crystalline cellulose. In contrast, primary wall cellulose levels remain unchanged in both mutants as measured in etiolated tbr and tbl3 hypocotyls, while the amount of esterified pectins is reduced and pectin methylesterase activity is increased in this tissue. Furthermore, etiolated tbr hypocotyls have reduced length with swollen epidermal cells, a phenotype characteristic for primary cesa mutants or the wild type treated with cellulose synthesis inhibitors. Taken together, we show that two TBL genes contribute to the synthesis and deposition of secondary wall cellulose, presumably by influencing the esterification state of pectic polymers. © 2010 American Society of Plant Biologists.
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