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פותח על ידי קלירמאש פתרונות בע"מ -
Manipulating volatile emission in tobacco leaves by expressing Aspergillus niger β-glucosidase in different subcellular compartments
Year:
2004
Source of publication :
Plant Biotechnology Journal
Authors :
לוינסון, אפרים
;
.
Volume :
2
Co-Authors:
Wei, S., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel, Horticulture Institute, Jiangsu Acad. of Agric. Sciences, Nanjing 210014, China
Marton, I., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Dekel, M., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Shalitin, D., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel, Dept. Molec., Cell and Devmtl. Biol., University of California, Los Angeles, CA 90024, United States
Lewinsohn, E., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel
Bravdo, B.-A., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Shoseyov, O., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Facilitators :
From page:
341
To page:
350
(
Total pages:
10
)
Abstract:
Expression of the Aspergillus niger β-glucosidase gene, BGL1, in Nicotiana tabacum plants (cv. Xanthi) had a profound effect on the volatile emissions of intact and crushed leaves. BGL1 was expressed under the control of the cauliflower mosaic virus (CaMV) 35S promoter and targeted to the cytoplasm, cell wall, lytic vacuole (LV), chloroplast or endoplasmic reticulum (ER). Subcellular localization was confirmed by gold immunolabelling, followed by transmission electron microscopy (TEM). Significant β-glucosidase activity was observed in transgenic plants expressing BGL1 in the cell wall, LV and ER. Compared with controls, all intact transgenic leaves were found to emit increased levels of 2-ethylhexanol, as determined by gas chromatography-mass spectrometry (GC-MS) analysis of the headspace volatiles. Plants expressing BGL1 in the cell wall (Tcw) emitted more trans-caryophyllene than did non-transgenic controls, whereas plants expressing BGL1 in the ER (Ter) and LV (Tvc) emitted more cembrene than did non-transgenic controls. Volatiles released from crushed transgenic leaves and collected with solid-phase microextraction (SPME) polydimethylsiloxane fibre were distinctly enhanced. Significant increases in linalool, nerol, furanoid cis-linalool oxide, 4-methyl-1-pentanol, 6-methyl-hept-5-en-2-ol and 2-ethylhexanol were detected in transgenic plants when compared with wild-type controls. 3-Hydroxyl-β-ionone levels were increased in crushed Tcw and Ter leaves, but were undetectable in Tvc leaves. The addition of glucoimidazole, a β-glucosidase inhibitor, abolished the increased emission of these volatiles. These results indicate that the expression of a fungal β-glucosidase gene in different subcellular compartments has the potential to affect the emission of plant volatiles, and thereby to modify plant-environment communication and aroma of agricultural products.
Note:
Related Files :
Aspergillus
Cauliflower mosaic virus
Glycosides
Nicotiana
Nicotiana tabacum
Volatiles
β-glucosidase
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1467-7652.2004.00077.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31017
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:59
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Scientific Publication
Manipulating volatile emission in tobacco leaves by expressing Aspergillus niger β-glucosidase in different subcellular compartments
2
Wei, S., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel, Horticulture Institute, Jiangsu Acad. of Agric. Sciences, Nanjing 210014, China
Marton, I., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Dekel, M., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Shalitin, D., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel, Dept. Molec., Cell and Devmtl. Biol., University of California, Los Angeles, CA 90024, United States
Lewinsohn, E., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel
Bravdo, B.-A., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Shoseyov, O., Inst. Plant Sci. Genet. in Agric., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Manipulating volatile emission in tobacco leaves by expressing Aspergillus niger β-glucosidase in different subcellular compartments
Expression of the Aspergillus niger β-glucosidase gene, BGL1, in Nicotiana tabacum plants (cv. Xanthi) had a profound effect on the volatile emissions of intact and crushed leaves. BGL1 was expressed under the control of the cauliflower mosaic virus (CaMV) 35S promoter and targeted to the cytoplasm, cell wall, lytic vacuole (LV), chloroplast or endoplasmic reticulum (ER). Subcellular localization was confirmed by gold immunolabelling, followed by transmission electron microscopy (TEM). Significant β-glucosidase activity was observed in transgenic plants expressing BGL1 in the cell wall, LV and ER. Compared with controls, all intact transgenic leaves were found to emit increased levels of 2-ethylhexanol, as determined by gas chromatography-mass spectrometry (GC-MS) analysis of the headspace volatiles. Plants expressing BGL1 in the cell wall (Tcw) emitted more trans-caryophyllene than did non-transgenic controls, whereas plants expressing BGL1 in the ER (Ter) and LV (Tvc) emitted more cembrene than did non-transgenic controls. Volatiles released from crushed transgenic leaves and collected with solid-phase microextraction (SPME) polydimethylsiloxane fibre were distinctly enhanced. Significant increases in linalool, nerol, furanoid cis-linalool oxide, 4-methyl-1-pentanol, 6-methyl-hept-5-en-2-ol and 2-ethylhexanol were detected in transgenic plants when compared with wild-type controls. 3-Hydroxyl-β-ionone levels were increased in crushed Tcw and Ter leaves, but were undetectable in Tvc leaves. The addition of glucoimidazole, a β-glucosidase inhibitor, abolished the increased emission of these volatiles. These results indicate that the expression of a fungal β-glucosidase gene in different subcellular compartments has the potential to affect the emission of plant volatiles, and thereby to modify plant-environment communication and aroma of agricultural products.
Scientific Publication
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