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Pectate lyase of Colletotrichum gloeosporioides attacking avocado fruits: cDNA cloning and involvement in pathogenicity
Year:
1997
Authors :
ותד, קסטרו
;
.
פרוסקי, דב
;
.
Volume :
50
Co-Authors:
Wattad, C., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Kobiler, D., Israel Inst. for Biological Research, Ness Ziona 40750, Israel
Dinoor, A., Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Prusky, D., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
197
To page:
212
(
Total pages:
16
)
Abstract:
Polyclonal antibodies were used to study the secretion of a pectate lyase: PLj during infection of avocado fruits by Colletotrichum gloeosporioides. Pectate lyase was detected in decayed fruit tissue 5 days after fruit inoculation, concomitantly with the decrease of the preformed antifungal 1-acetoxy-2-hydroxy-4-oxo-12,15-heneicosa diene to subfungitoxic concentrations. A higher amount of the enzyme was detected in the leading edge of the decayed lesion than in the already decayed tissue. As a first stage to determine the relation between PLs form C. gloeosporioides attacking avocado and PLs from Colletotrichum strains attacking other fruits, a cDNA encoding PL of C. gloeosporioides was cloned and sequenced. The cloned DNA showed high homology to known pels from fungi, bacteria and plants. The pel gene of C. gloeosporioides hybridized to single genomic restriction fragments from C. gloeosporioides isolated from mango and papaya fruits and Colletotrichum musae from banana fruit. Western blot analysis of culture fluids from these pathogens revealed one band of 28 41 kDa that specifically cross-reacted with antibodies raised against the pectate lyase from C. gloeosporioides isolated from avocado. Pectate lyase antibodies had no effect on spore germination, germ tube elongation or appressoria formation. However, when conidia were mixed with the antibodies prior to inoculation, symptom development was inhibited on avocado, mango and banana fruits. These results suggest the presence of a similar PL in Colletotrichum strains attacking avocado, mango, papaya and banana. The detection and inhibition of fungal development by PL antibodies suggest the importance of this enzyme during C. gloeosporioides attack in fruits.
Note:
Related Files :
Colletotrichum gloeosporioides
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/pmpp.1997.0080
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31029
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:59
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Scientific Publication
Pectate lyase of Colletotrichum gloeosporioides attacking avocado fruits: cDNA cloning and involvement in pathogenicity
50
Wattad, C., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Kobiler, D., Israel Inst. for Biological Research, Ness Ziona 40750, Israel
Dinoor, A., Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Prusky, D., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Pectate lyase of Colletotrichum gloeosporioides attacking avocado fruits: cDNA cloning and involvement in pathogenicity
Polyclonal antibodies were used to study the secretion of a pectate lyase: PLj during infection of avocado fruits by Colletotrichum gloeosporioides. Pectate lyase was detected in decayed fruit tissue 5 days after fruit inoculation, concomitantly with the decrease of the preformed antifungal 1-acetoxy-2-hydroxy-4-oxo-12,15-heneicosa diene to subfungitoxic concentrations. A higher amount of the enzyme was detected in the leading edge of the decayed lesion than in the already decayed tissue. As a first stage to determine the relation between PLs form C. gloeosporioides attacking avocado and PLs from Colletotrichum strains attacking other fruits, a cDNA encoding PL of C. gloeosporioides was cloned and sequenced. The cloned DNA showed high homology to known pels from fungi, bacteria and plants. The pel gene of C. gloeosporioides hybridized to single genomic restriction fragments from C. gloeosporioides isolated from mango and papaya fruits and Colletotrichum musae from banana fruit. Western blot analysis of culture fluids from these pathogens revealed one band of 28 41 kDa that specifically cross-reacted with antibodies raised against the pectate lyase from C. gloeosporioides isolated from avocado. Pectate lyase antibodies had no effect on spore germination, germ tube elongation or appressoria formation. However, when conidia were mixed with the antibodies prior to inoculation, symptom development was inhibited on avocado, mango and banana fruits. These results suggest the presence of a similar PL in Colletotrichum strains attacking avocado, mango, papaya and banana. The detection and inhibition of fungal development by PL antibodies suggest the importance of this enzyme during C. gloeosporioides attack in fruits.
Scientific Publication
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