אסיף מאגר המחקר החקלאי

Identification and classification of the Spodoptera littoralis nucleopolyhedrovirus inhibitor of apoptosis gene

Year:

2003

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Volume :

26

Co-Authors:

Liu, Q., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel, Institute of Virology, Wuhan University, Wuhan 430072, China
Qi, Y., Institute of Virology, Wuhan University, Wuhan 430072, China
Chejanovsky, N., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel

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Abstract:

Baculoviruses possess two types of genes that suppressed apoptosis, p35 and inhibitor of apoptosis (iap). In this study we report the isolation and identification of an inhibitor of apoptosis gene Sliap in the genome of the Spodoptera littoralis nucleopolyhedrovirus (SlNPV). The Sliap sequence predicted a 15 kDa polypeptide with only one BIR domain and a RING finger, both motifs characteristic of the IAP family of proteins, and a third specific acidic-rich motif. These characteristics, shared with the Spodoptera littura NPV IAP2/3, Epiphyas postvittana NPV IAP4, Lymantria dispar NPV IAP and Orgyia pseudotsugata NPV IAP4 (Orf 107) allowed us to classify them in a new homology group (IAP-4). Sliap was able to delay, but not to suppress, apoptosis induced by replication of a recombinant AcMNPV deficient in p35. In SlNPV infected-SF9 cells Sliap was expressed earlier than sl-p49 suggesting that its role at the initiation of infection was to delay the apoptotic response of the host.

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