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פותח על ידי קלירמאש פתרונות בע"מ -
Application of a locus-specific DNA hybridization probe in the analysis of the slow-feathering endogenous virus complex of chickens.
Year:
1991
Source of publication :
Poultry Science
Authors :
לוין, אילן
;
.
Volume :
70
Co-Authors:
Smith, E.J., U.S. Department of Agriculture, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Levin, I., U.S. Department of Agriculture, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Facilitators :
From page:
1957
To page:
1964
(
Total pages:
8
)
Abstract:
To investigate further the sex-linked, slow-feathering (SF) locus, a DNA hybridization probe that flanks the integration site of endogenous virus ev21 was used to probe Southern blots from a variety of commercial SF White Leghorn (WL), broiler, and endangered lines. After HaeIII digestion of DNA from SF WL, the 5' and 3' provirus-cell junction fragments were seen in addition to a 2.5-kb fragment of cell sequences that was homologous with the viral integration region. The latter polymorphism, which appeared to represent sequences duplicated after integration of EV21, was designated unoccupied repeat URa. Among SF broiler crosses, the same provirus-cell junction fragments were found but the pristine region that represented the 1.6-kb proviral URb was also found. Only URb was found among rapid-feathering (RF) chickens, regardless of breed. Although there was marked (greater than 95%), agreement between the presence of ev21-cell; junction fragments and the SF phenotype among both WL and broilers, Southern blots of DNA from a few commercial SF broiler chickens lacked ev21 junction fragments but some RF revertants harbored ev21 junction fragments. These anomalies suggest that ev21 may not be the sole determinant of the SF phenotype.
Note:
Related Files :
Animal
DNA
Feathers
Female
Genetics
Growth, Development and Aging
Leukosis Virus, Avian
Male
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31102
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:59
Scientific Publication
Application of a locus-specific DNA hybridization probe in the analysis of the slow-feathering endogenous virus complex of chickens.
70
Smith, E.J., U.S. Department of Agriculture, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Levin, I., U.S. Department of Agriculture, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Application of a locus-specific DNA hybridization probe in the analysis of the slow-feathering endogenous virus complex of chickens.
To investigate further the sex-linked, slow-feathering (SF) locus, a DNA hybridization probe that flanks the integration site of endogenous virus ev21 was used to probe Southern blots from a variety of commercial SF White Leghorn (WL), broiler, and endangered lines. After HaeIII digestion of DNA from SF WL, the 5' and 3' provirus-cell junction fragments were seen in addition to a 2.5-kb fragment of cell sequences that was homologous with the viral integration region. The latter polymorphism, which appeared to represent sequences duplicated after integration of EV21, was designated unoccupied repeat URa. Among SF broiler crosses, the same provirus-cell junction fragments were found but the pristine region that represented the 1.6-kb proviral URb was also found. Only URb was found among rapid-feathering (RF) chickens, regardless of breed. Although there was marked (greater than 95%), agreement between the presence of ev21-cell; junction fragments and the SF phenotype among both WL and broilers, Southern blots of DNA from a few commercial SF broiler chickens lacked ev21 junction fragments but some RF revertants harbored ev21 junction fragments. These anomalies suggest that ev21 may not be the sole determinant of the SF phenotype.
Scientific Publication
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