Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis

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Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis

Year: 

1999

Source of publication :

Genome

Authors :

גרמה, אדריאנה

.

Volume :

Co-Authors: 

Chagué, V., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel, Inst. de Biotechnologie des Plantes, Bâtiment 630, Universite Paris-Sud, 91405 Orsay Cedex, France
Fahima, T., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel
Dahan, A., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel
Sun, G.L., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel, Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City 611830 Sichuan, China
Korol, A.B., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel
Ronin, Y.I., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel
Grama, A., Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Röder, M.S., Inst. for Plant Genet./Crop Plant R., D-06466 Gatersleben, Germany
Nevo, E., Institute of Evolution, University of Haifa, Mount Camel, Haifa 31905, Israel

From page: 

1050

To page: 

1056

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Total pages: 

7

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Link :

Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis

Abstract: 

Microsatellite and random amplified polymorphic DNA (RAPD) primers were used to identify molecular markers linked to the Yr15 gene which confer resistance to stripe rust (Puccina striiformis Westend) in wheat. By using near isogenic lines (NILs) for the Yr15 gene and a F2 mapping population derived from crosses of these lines and phenotyped for resistance, we identified one microsatellite marker (GWM33) and one RAPD marker (OPA19800) linked to Yr15. Then, bulked segregant analysis was used in addition to the NILs to identify RAPD markers linked to the target gene. Using this approach, two RAPD markers linked to Yr15 were identified, one in coupling (UBC199700) and one in repulsion phase (UBC2121200). After MAPMAKER linkage analysis on the F2 population, the two closest markers were shown to be linked to Yr15 within a distance of about 12 cM. The recombination rates were recalculated using the maximum likelihood technique to take into account putative escaped individuals from the stripe rust resistance test and obtain unbiased distance estimates. As a result of this study, the stripe rust resistance gene Yr15 is surrounded by two flanking PCR markers, UBC199700 and GWM33, at about 5 cM from each side.