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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of endothelin-1 and nitric oxide generating systems in corpus luteum-derived endothelial cells
Year:
2004
Source of publication :
Reproduction
Authors :
קיסיליוק, טטיאנה
;
.
Volume :
128
Co-Authors:
Klipper, E., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Gilboa, T., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Levy, N., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Kisliouk, T., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Spanel-Borowski, K., Institute of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig, Germany
Meidan, R., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
463
To page:
473
(
Total pages:
11
)
Abstract:
Endothelium-derived endothelin-1 (ET-1) and nitric oxide (NO) are pivotal regulators of corpus luteum (CL) function. To have a better insight into their synthesis and action, members of the ET system (ET-1, ET converting enzyme (ECE-1) isoforms a-d, ETA and ETB receptors) along with NO synthase (NOS) isoforms - endothelial (e)NOS and inducible (i)NOS - were quantified in CL-derived endothelial cells (CLEC). The expression of these genes in microvascular CLEC, obtained by lectin-coated magnetic beads, was compared with cells removed from the luteal microenvironment and maintained in culture for different durations, and with endothelial cells (EC) derived from a large blood vessel (i.e. bovine aortic endothelial cells, BAEC). The profile of gene expression in the different EC types was determined by quantitative real-time PCR. Freshly isolated EC from mid-cycle CL exhibited high ET-1 receptor expression (both ETA and ETB), low ET-1 synthesizing ability (both prepro (pp) ET-1 and ECE-1), but elevated iNOS - the high throughput NOS isoform. The distinct phenotype of CLEC was lost soon after an overnight culture. ETA and ETB receptor levels declined, ppET-1 levels increased while iNOS was reduced. These changes were extenuated during long-term culture of CLEC. The general pattern of gene expression in BAEC and long-term cultured CLEC was similar yet some differences, reminiscent of freshly isolated CLEC, remained: ECE-1c, ETB receptor and NOS isoforms were expressed differently in BAEC as compared with lines of CLEC. This study suggests that the luteal microenvironment is necessary to sustain the selective phenotype of its resident endothelial cells. The inverse relationship between ppET-1 and iNOS observed in freshly isolated CLEC and in cultured cells is physiologically significant and suggests that ET-1 and NO may modulate the production of each other. © 2004 Society for Reproduction and Fertility.
Note:
Related Files :
animal cell
Animals
Antigens, CD31
cattle
corpus luteum
Female
Metalloendopeptidases
phenotypic variation
Receptor, TIE-2
עוד תגיות
תוכן קשור
More details
DOI :
10.1530/rep.1.00271
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31255
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
You may also be interested in
Scientific Publication
Characterization of endothelin-1 and nitric oxide generating systems in corpus luteum-derived endothelial cells
128
Klipper, E., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Gilboa, T., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Levy, N., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Kisliouk, T., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Spanel-Borowski, K., Institute of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig, Germany
Meidan, R., Department of Animal Sciences, Fac. of Agric. Food/Environ. Quality, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Characterization of endothelin-1 and nitric oxide generating systems in corpus luteum-derived endothelial cells
Endothelium-derived endothelin-1 (ET-1) and nitric oxide (NO) are pivotal regulators of corpus luteum (CL) function. To have a better insight into their synthesis and action, members of the ET system (ET-1, ET converting enzyme (ECE-1) isoforms a-d, ETA and ETB receptors) along with NO synthase (NOS) isoforms - endothelial (e)NOS and inducible (i)NOS - were quantified in CL-derived endothelial cells (CLEC). The expression of these genes in microvascular CLEC, obtained by lectin-coated magnetic beads, was compared with cells removed from the luteal microenvironment and maintained in culture for different durations, and with endothelial cells (EC) derived from a large blood vessel (i.e. bovine aortic endothelial cells, BAEC). The profile of gene expression in the different EC types was determined by quantitative real-time PCR. Freshly isolated EC from mid-cycle CL exhibited high ET-1 receptor expression (both ETA and ETB), low ET-1 synthesizing ability (both prepro (pp) ET-1 and ECE-1), but elevated iNOS - the high throughput NOS isoform. The distinct phenotype of CLEC was lost soon after an overnight culture. ETA and ETB receptor levels declined, ppET-1 levels increased while iNOS was reduced. These changes were extenuated during long-term culture of CLEC. The general pattern of gene expression in BAEC and long-term cultured CLEC was similar yet some differences, reminiscent of freshly isolated CLEC, remained: ECE-1c, ETB receptor and NOS isoforms were expressed differently in BAEC as compared with lines of CLEC. This study suggests that the luteal microenvironment is necessary to sustain the selective phenotype of its resident endothelial cells. The inverse relationship between ppET-1 and iNOS observed in freshly isolated CLEC and in cultured cells is physiologically significant and suggests that ET-1 and NO may modulate the production of each other. © 2004 Society for Reproduction and Fertility.
Scientific Publication
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