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Plant Journal
Damodharan, S., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, Israel
Zhao, D., Department of Biological Sciences, University of Wisconsin-Milwaukee, Lapham Hall S181, 3209 N. Maryland Avenue, Milwaukee, WI, United States
Arazi, T., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, Israel
Damodharan, S., Institute of Plant Sciences Agricultural Research Organization Volcani Center PO Box 6 Bet Dagan 50250 Israel
Zhao, D., Department of Biological Sciences University of Wisconsin-Milwaukee Lapham Hall S181 3209 N. Maryland Avenue Milwaukee 53201-0413WI USA
Arazi, T., Institute of Plant Sciences Agricultural Research Organization Volcani Center PO Box 6 Bet Dagan 50250 Israel
Plant microRNAs play vital roles in auxin signaling via the negative regulation of auxin response factors (ARFs). Studies have shown that targeting of ARF10/16/17 by miR160 is indispensable for various aspects of development, but its functions in the model crop tomato (Solanum lycopersicum) are unknown. Here we knocked down miR160 (sly–miR160) using a short tandem target mimic (STTM160), and investigated its roles in tomato development. Northern blot analysis showed that miR160 is abundant in developing ovaries. In line with this, its down-regulation perturbed ovary patterning as indicated by the excessive elongation of the proximal ends of mutant ovaries and thinning of the placenta. Following fertilization, these morphological changes led to formation of elongated, pear-shaped fruits reminiscent of those of the tomato ovate mutant. In addition, STTM160-expressing plants displayed abnormal floral organ abscission, and produced leaves, sepals and petals with diminished blades, indicating a requirement for sly–miR160 for these auxin-mediated processes. We found that sly–miR160 depletion was always associated with the up-regulation of SlARF10A, SlARF10B and SlARF17, of which the expression of SlARF10A increased the most. Despite the sly–miR160 legitimate site of SlARF16A, its mRNA levels did not change in response to sly–miR160 down-regulation, suggesting that it may be regulated by a mechanism other than mRNA cleavage. SlARF10A and SlARF17 were previously suggested to function as inhibiting ARFs. We propose that by adjusting the expression of a group of ARF repressors, of which SlARF10A is a primary target, sly–miR160 regulates auxin-mediated ovary patterning as well as floral organ abscission and lateral organ lamina outgrowth. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd
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A common miRNA160-based mechanism regulates ovary patterning, floral organ abscission and lamina outgrowth in tomato
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Damodharan, S., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, Israel
Zhao, D., Department of Biological Sciences, University of Wisconsin-Milwaukee, Lapham Hall S181, 3209 N. Maryland Avenue, Milwaukee, WI, United States
Arazi, T., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, Israel
Damodharan, S., Institute of Plant Sciences Agricultural Research Organization Volcani Center PO Box 6 Bet Dagan 50250 Israel
Zhao, D., Department of Biological Sciences University of Wisconsin-Milwaukee Lapham Hall S181 3209 N. Maryland Avenue Milwaukee 53201-0413WI USA
Arazi, T., Institute of Plant Sciences Agricultural Research Organization Volcani Center PO Box 6 Bet Dagan 50250 Israel
A common miRNA160-based mechanism regulates ovary patterning, floral organ abscission and lamina outgrowth in tomato
Plant microRNAs play vital roles in auxin signaling via the negative regulation of auxin response factors (ARFs). Studies have shown that targeting of ARF10/16/17 by miR160 is indispensable for various aspects of development, but its functions in the model crop tomato (Solanum lycopersicum) are unknown. Here we knocked down miR160 (sly–miR160) using a short tandem target mimic (STTM160), and investigated its roles in tomato development. Northern blot analysis showed that miR160 is abundant in developing ovaries. In line with this, its down-regulation perturbed ovary patterning as indicated by the excessive elongation of the proximal ends of mutant ovaries and thinning of the placenta. Following fertilization, these morphological changes led to formation of elongated, pear-shaped fruits reminiscent of those of the tomato ovate mutant. In addition, STTM160-expressing plants displayed abnormal floral organ abscission, and produced leaves, sepals and petals with diminished blades, indicating a requirement for sly–miR160 for these auxin-mediated processes. We found that sly–miR160 depletion was always associated with the up-regulation of SlARF10A, SlARF10B and SlARF17, of which the expression of SlARF10A increased the most. Despite the sly–miR160 legitimate site of SlARF16A, its mRNA levels did not change in response to sly–miR160 down-regulation, suggesting that it may be regulated by a mechanism other than mRNA cleavage. SlARF10A and SlARF17 were previously suggested to function as inhibiting ARFs. We propose that by adjusting the expression of a group of ARF repressors, of which SlARF10A is a primary target, sly–miR160 regulates auxin-mediated ovary patterning as well as floral organ abscission and lateral organ lamina outgrowth. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd
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