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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a primary in vivo degradation product of the rapidly-turning-over 32 kd protein of photosystem II.
Year:
1987
Source of publication :
eLife
Authors :
גאבה, ויקטור
;
.
Volume :
6
Co-Authors:
Greenberg, B.M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Gaba, V., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Mattoo, A.K., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Edelman, M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Facilitators :
From page:
2865
To page:
2869
(
Total pages:
5
)
Abstract:
The 32 kd photosystem II protein of plant chloroplasts is rapidly turned over in the light. The initial events in the degradation of the 32 kd protein were studied. A 23.5 kd breakdown product was identified in Spirodela oligorrhiza membranes using immunological analysis. The 23.5 kd polypeptide was shown to be derived from the amino-terminal portion of the 32 kd protein using partial proteolytic fingerprinting. An in vivo precursor--product relationship between the 32 kd protein and the 23.5 kd polypeptide was kinetically demonstrated by radiolabeling and pulse-chase experiments. The cleavage site yielding the 23.5 kd polypeptide was localized to a functionally active region (between helices IV and V) of the 32 kd protein. We propose that an alpha-helix-destabilizing 'degradation' sequence, bordered by arginine residues 225 and 238, is involved in the formation of the 23.5 kd polypeptide.
Note:
Related Files :
chlorophyll
Chloroplasts
Kinetics
light
Macromolecular Systems
metabolism
photosynthesis
plant
Plants
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31329
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
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Scientific Publication
Identification of a primary in vivo degradation product of the rapidly-turning-over 32 kd protein of photosystem II.
6
Greenberg, B.M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Gaba, V., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Mattoo, A.K., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Edelman, M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Identification of a primary in vivo degradation product of the rapidly-turning-over 32 kd protein of photosystem II.
The 32 kd photosystem II protein of plant chloroplasts is rapidly turned over in the light. The initial events in the degradation of the 32 kd protein were studied. A 23.5 kd breakdown product was identified in Spirodela oligorrhiza membranes using immunological analysis. The 23.5 kd polypeptide was shown to be derived from the amino-terminal portion of the 32 kd protein using partial proteolytic fingerprinting. An in vivo precursor--product relationship between the 32 kd protein and the 23.5 kd polypeptide was kinetically demonstrated by radiolabeling and pulse-chase experiments. The cleavage site yielding the 23.5 kd polypeptide was localized to a functionally active region (between helices IV and V) of the 32 kd protein. We propose that an alpha-helix-destabilizing 'degradation' sequence, bordered by arginine residues 225 and 238, is involved in the formation of the 23.5 kd polypeptide.
Scientific Publication
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