Co-Authors:
Spiegel-Roy, P., Department of Fruit Breeding and Genetics, Volcani Center, A.R.O. Bet Dagan P.O.B. 6, Israel
Saad, S., Department of Fruit Breeding and Genetics, Volcani Center, A.R.O. Bet Dagan P.O.B. 6, Israel
Abstract:
Embryogenesis in callus lines of Citrus sinensis (orange) selected for tolerance to NaCl stress was compared to an unselected line. Addition of inhibitors of gibberellic acid (GA3) synthesis, 2.6 × 10-6M paclobutrazol or 6.2 × 10-6M daminozide (butanedioic acid mono-(2-2-dimethylhydrazide) to the medium enhanced embryogenesis in the unselected line and two of the three salt tolerant lines, and also overcame embryo browning in the salt tolerant line R13. Sucrose as a carbon source was generally ineffective for embryogenesis. Two percent (w/v) glycerol was more effective than 2% (w/v) galactose, especially in older cultures. Formation of embryos was quicker in the non-selected line. Only a slight improvement in embryogenic potential was noted upon removal of salt tolerant lines for three passages from NaCl containing medium to basal medium without salt. © 1986.