Effect of carbohydrates and inhibitors of GA3 biosynthesis on embryogenenic potential of salt tolerant and non-tolerant callus lines of orange (Citrus sinensis osbeck)

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פותח על ידי קלירמאש פתרונות בע"מ -

Effect of carbohydrates and inhibitors of GA3 biosynthesis on embryogenenic potential of salt tolerant and non-tolerant callus lines of orange (Citrus sinensis osbeck)

Year: 

1986

Source of publication :

Plant Science

Authors :

סעד, שושנה

;

שפיגל-רואי, פנחס

.

Volume :

Co-Authors: 

Spiegel-Roy, P., Department of Fruit Breeding and Genetics, Volcani Center, A.R.O. Bet Dagan P.O.B. 6, Israel
Saad, S., Department of Fruit Breeding and Genetics, Volcani Center, A.R.O. Bet Dagan P.O.B. 6, Israel

From page: 

215

To page: 

220

(

Total pages: 

6

)

Link :

Effect of carbohydrates and inhibitors of GA3 biosynthesis on embryogenenic potential of salt tolerant and non-tolerant callus lines of orange (Citrus sinensis osbeck)

Abstract: 

Embryogenesis in callus lines of Citrus sinensis (orange) selected for tolerance to NaCl stress was compared to an unselected line. Addition of inhibitors of gibberellic acid (GA3) synthesis, 2.6 × 10-6M paclobutrazol or 6.2 × 10-6M daminozide (butanedioic acid mono-(2-2-dimethylhydrazide) to the medium enhanced embryogenesis in the unselected line and two of the three salt tolerant lines, and also overcame embryo browning in the salt tolerant line R13. Sucrose as a carbon source was generally ineffective for embryogenesis. Two percent (w/v) glycerol was more effective than 2% (w/v) galactose, especially in older cultures. Formation of embryos was quicker in the non-selected line. Only a slight improvement in embryogenic potential was noted upon removal of salt tolerant lines for three passages from NaCl containing medium to basal medium without salt. © 1986.