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פותח על ידי קלירמאש פתרונות בע"מ -
Genomic and phenomic study of mammary pathogenic Escherichia coli
Year:
2015
Source of publication :
PLoS ONE
Authors :
סלע, שלמה
;
.
Volume :
10
Co-Authors:
Blum, S.E., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel, National Mastitis Reference Center, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Heller, E.D., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Volcani Center, ARO, Bet Dagan, Israel
Elad, D., Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Edery, N., Department of Pathology, Kimron Veterinary Institute, Bet Dagan, Israel
Leitner, G., National Mastitis Reference Center, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Escherichia coli is a major etiological agent of intra-mammary infections (IMI) in cows, leading to acute mastitis and causing great economic losses in dairy production worldwide. Particular strains cause persistent IMI, leading to recurrent mastitis. Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis are largely unknown. This study aimed to identify virulence markers in MPEC through whole genome and phenome comparative analysis. MPEC strains causing acute (VL2874 and P4) or persistent (VL2732) mastitis were compared to an environmental strain (K71) and to the genomes of strains representing different E. coli pathotypes. Intra-mammary challenge in mice confirmed experimentally that the strains studied here have different pathogenic potential, and that the environmental strain K71 is non-pathogenic in the mammary gland. Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes. Functional features identified in MPEC genomes and lacking in the non-mammary pathogenic strain were associated with synthesis of lipopolysaccharide and other membrane antigens, ferric-dicitrate iron acquisition and sugars metabolism. Features associated with cytotoxicity or intra-cellular survival were found specifically in the genomes of strains from severe and acute (VL2874) or persistent (VL2732) mastitis, respectively. MPEC genomes were relatively similar to strain K-12, which was subsequently shown here to be possibly pathogenic in the mammary gland. Phenome analysis showed that the persistent MPEC was the most versatile in terms of nutrients metabolized and acute MPEC the least. Among phenotypes unique to MPEC compared to the non-mammary pathogenic strain were uric acid and D-serine metabolism. This study reveals virulence factors and phenotypic characteristics of MPEC that may play a role in pathogenesis of E. coli mastitis. Copyright: © 2015 Blum et al.
Note:
Related Files :
animal cell
animal experiment
animal model
animal tissue
Female
genomics
phenotype
עוד תגיות
תוכן קשור
More details
DOI :
10.1371/journal.pone.0136387
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31379
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
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Scientific Publication
Genomic and phenomic study of mammary pathogenic Escherichia coli
10
Blum, S.E., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel, National Mastitis Reference Center, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Heller, E.D., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Volcani Center, ARO, Bet Dagan, Israel
Elad, D., Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Edery, N., Department of Pathology, Kimron Veterinary Institute, Bet Dagan, Israel
Leitner, G., National Mastitis Reference Center, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Genomic and phenomic study of mammary pathogenic Escherichia coli
Escherichia coli is a major etiological agent of intra-mammary infections (IMI) in cows, leading to acute mastitis and causing great economic losses in dairy production worldwide. Particular strains cause persistent IMI, leading to recurrent mastitis. Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis are largely unknown. This study aimed to identify virulence markers in MPEC through whole genome and phenome comparative analysis. MPEC strains causing acute (VL2874 and P4) or persistent (VL2732) mastitis were compared to an environmental strain (K71) and to the genomes of strains representing different E. coli pathotypes. Intra-mammary challenge in mice confirmed experimentally that the strains studied here have different pathogenic potential, and that the environmental strain K71 is non-pathogenic in the mammary gland. Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes. Functional features identified in MPEC genomes and lacking in the non-mammary pathogenic strain were associated with synthesis of lipopolysaccharide and other membrane antigens, ferric-dicitrate iron acquisition and sugars metabolism. Features associated with cytotoxicity or intra-cellular survival were found specifically in the genomes of strains from severe and acute (VL2874) or persistent (VL2732) mastitis, respectively. MPEC genomes were relatively similar to strain K-12, which was subsequently shown here to be possibly pathogenic in the mammary gland. Phenome analysis showed that the persistent MPEC was the most versatile in terms of nutrients metabolized and acute MPEC the least. Among phenotypes unique to MPEC compared to the non-mammary pathogenic strain were uric acid and D-serine metabolism. This study reveals virulence factors and phenotypic characteristics of MPEC that may play a role in pathogenesis of E. coli mastitis. Copyright: © 2015 Blum et al.
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