נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons
Year:
1999
Authors :
אלמן, חסיה
;
.
גאבה, ויקטור
;
.
ותד, עבד
;
.
Volume :
35
Co-Authors:
Gaba, V., Department Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Schlarman, E., Department Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Elman, C., Department Citriculture, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Sagee, O., Department Citriculture, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Watad, A.A., Department Floriculture, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Gray, D.J., IFAS, CFREC, University of Florida, 5523 University Avenue, Leesburg, FL 34748-8232, United States
Facilitators :
From page:
1
To page:
7
(
Total pages:
7
)
Abstract:
The anatomy and morphology of bud regeneration were investigated in melon (Cucumis melo L.) cv. Galia, which regenerates in vitro only by direct organogenesis from the cotyledon explant. Explants were cut from the cotyledon proximal to the apex from 3-d-old in vitro seedlings. After 3 d on Murashige and Skoog medium with N6-benzyladenine, cell division can be observed in the epidermal layer on the adaxial side in the center of the explant, near the most proximal (wounded) cut edge. Over the next week, the area of the meristem increases laterally. Additional cell layers are added to the meristematic area by cell division in the epidermis. In places the epidermis remains active in cell division. Alongside those active areas there are zones where the epidermis has become inactive, although the subepidermal layers continue to divide. In transverse section, the explant now has small protuberances on the adaxial surface. After 10 d on cytokinin-containing medium, the first signs of development are visible on the adaxial surface adjacent to the proximal cut edge. The protuberances observed after 10 d are neither primordia nor buds, although some meristematic bulges are observed. The first regenerated shoot buds are observed histologically after 15 d, by which time the surface has many protuberances and some small leaves. The first shoot is found by histology after 22 d. By this time the surface is covered with protrusions and leaves, mostly without accompanying buds. The leaves may be produced from the protrusions initially visible after 10 d.
Note:
Related Files :
Cucumis melo
Direct organogenesis
Histology
Leaf primordia
organogenesis
Shoot buds
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31436
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:02
Scientific Publication
In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons
35
Gaba, V., Department Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Schlarman, E., Department Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Elman, C., Department Citriculture, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Sagee, O., Department Citriculture, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Watad, A.A., Department Floriculture, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Gray, D.J., IFAS, CFREC, University of Florida, 5523 University Avenue, Leesburg, FL 34748-8232, United States
In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons
The anatomy and morphology of bud regeneration were investigated in melon (Cucumis melo L.) cv. Galia, which regenerates in vitro only by direct organogenesis from the cotyledon explant. Explants were cut from the cotyledon proximal to the apex from 3-d-old in vitro seedlings. After 3 d on Murashige and Skoog medium with N6-benzyladenine, cell division can be observed in the epidermal layer on the adaxial side in the center of the explant, near the most proximal (wounded) cut edge. Over the next week, the area of the meristem increases laterally. Additional cell layers are added to the meristematic area by cell division in the epidermis. In places the epidermis remains active in cell division. Alongside those active areas there are zones where the epidermis has become inactive, although the subepidermal layers continue to divide. In transverse section, the explant now has small protuberances on the adaxial surface. After 10 d on cytokinin-containing medium, the first signs of development are visible on the adaxial surface adjacent to the proximal cut edge. The protuberances observed after 10 d are neither primordia nor buds, although some meristematic bulges are observed. The first regenerated shoot buds are observed histologically after 15 d, by which time the surface has many protuberances and some small leaves. The first shoot is found by histology after 22 d. By this time the surface is covered with protrusions and leaves, mostly without accompanying buds. The leaves may be produced from the protrusions initially visible after 10 d.
Scientific Publication
You may also be interested in