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פותח על ידי קלירמאש פתרונות בע"מ -
Genetic transformation of HeLa cells by Agrobacterium
Year:
2001
Authors :
גפני, ידידיה
;
.
קפולניק, יורם
;
.
Volume :
98
Co-Authors:
Kunik, T., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States, Institute of Field and Garden Crops, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel, Department of Pharmacology, State University of New York, Stony Brook, NY 11794-8651, United States
Tzfira, T., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Kapulnik, Y., Institute of Field and Garden Crops, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Gafni, Y., Institute of Field and Garden Crops, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Dingwall, C., Department of Pharmacology, State University of New York, Stony Brook, NY 11794-8651, United States, Neurosciences Research Department, SmithKline-Beecham Pharmaceuticals, Harlow, Essex, United Kingdom
Citovsky, V., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Facilitators :
From page:
1871
To page:
1876
(
Total pages:
6
)
Abstract:
Agrobacterium tumefaciens is a soil phytopathogen that elicits neoplastic growths on the host plant species. In nature, however, Agrobacterium also may encounter organisms belonging to other kingdoms such as insects and animals that feed on the infected plants. Can Agrobacterium, then, also infect animal cells? Here, we report that Agrobacterium attaches to and genetically transforms several types of human cells. In stably transformed HeLa cells, the integration event occurred at the right border of the tumor-inducing plasmid's transferred-DNA (T-DNA), suggesting bona fide T-DNA transfer and lending support to the notion that Agrobacterium transforms human cells by a mechanism similar to that which it uses for transformation of plants cells. Collectively, our results suggest that Agrobacterium can transport its T-DNA to human cells and integrate it into their genome.
Note:
Related Files :
Agrobacterium
Animals
DNA
genetic transformation
Genome
PC12 Cells
Plant Cell
Virulence Factors
עוד תגיות
תוכן קשור
More details
DOI :
10.1073/pnas.041327598
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31548
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:03
Scientific Publication
Genetic transformation of HeLa cells by Agrobacterium
98
Kunik, T., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States, Institute of Field and Garden Crops, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel, Department of Pharmacology, State University of New York, Stony Brook, NY 11794-8651, United States
Tzfira, T., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Kapulnik, Y., Institute of Field and Garden Crops, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Gafni, Y., Institute of Field and Garden Crops, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Dingwall, C., Department of Pharmacology, State University of New York, Stony Brook, NY 11794-8651, United States, Neurosciences Research Department, SmithKline-Beecham Pharmaceuticals, Harlow, Essex, United Kingdom
Citovsky, V., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Genetic transformation of HeLa cells by Agrobacterium
Agrobacterium tumefaciens is a soil phytopathogen that elicits neoplastic growths on the host plant species. In nature, however, Agrobacterium also may encounter organisms belonging to other kingdoms such as insects and animals that feed on the infected plants. Can Agrobacterium, then, also infect animal cells? Here, we report that Agrobacterium attaches to and genetically transforms several types of human cells. In stably transformed HeLa cells, the integration event occurred at the right border of the tumor-inducing plasmid's transferred-DNA (T-DNA), suggesting bona fide T-DNA transfer and lending support to the notion that Agrobacterium transforms human cells by a mechanism similar to that which it uses for transformation of plants cells. Collectively, our results suggest that Agrobacterium can transport its T-DNA to human cells and integrate it into their genome.
Scientific Publication
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