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פותח על ידי קלירמאש פתרונות בע"מ -
Restriction Fragment Length Polymorphism In Sheep at The Growth Hormone Locus is The Result of Variation In Gene Number
Year:
1990
Source of publication :
Animal Biotechnology
Authors :
גוטויין, אלישע
;
.
ולינסקי, עמוס
;
.
שני, משה
;
.
Volume :
1
Co-Authors:
Valinskv, A., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Gootwine, E., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
135
To page:
144
(
Total pages:
10
)
Abstract:
DNA samples from 70 sheep of the Awassi, Assaf, Booroola Merino and German Mutton Merino breeds, of the Booroola Merino X Awassi and Booroola Merino X Assaf crosses, of three wild sheep, and of seven Saanen goats, were digested with the restriction enzymes BamHI, EcoRI and HindIII, and screened for restriction fragment length polymorphism (RFLP) using the bovine growth hormone (GH) gene as a probe. This and further analysis of selected DNA samples with 6 additional restriction enzymes (EcoRV Pst I, PvuII, Rsal, Sacl and TaqI) revealed RFLP’s that is probably due to the presence of two alleles at the GH locus; A predominant allele (Gh2), in which the growth hormone gene is duplicated and the two copies are located at a distance of 3.5 Kb apart, and a rare allele (Ghl), in which the growth hormone gene is present as a single copy. The results also indicate that while the 5’ flanking regions of the two GH copies of the Gh2 allele are different, the 3’ flanking region appears to be conserved. © 1990, Taylor & Francis Group, LLC. All rights reserved.
Note:
Related Files :
ASSAF
Awassi
Booroola Merino
DNA
Genetics
goats
hormones
Saanen
sheep
עוד תגיות
תוכן קשור
More details
DOI :
10.1080/10495399009525736
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31560
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:03
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Scientific Publication
Restriction Fragment Length Polymorphism In Sheep at The Growth Hormone Locus is The Result of Variation In Gene Number
1
Valinskv, A., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Gootwine, E., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Restriction Fragment Length Polymorphism In Sheep at The Growth Hormone Locus is The Result of Variation In Gene Number
DNA samples from 70 sheep of the Awassi, Assaf, Booroola Merino and German Mutton Merino breeds, of the Booroola Merino X Awassi and Booroola Merino X Assaf crosses, of three wild sheep, and of seven Saanen goats, were digested with the restriction enzymes BamHI, EcoRI and HindIII, and screened for restriction fragment length polymorphism (RFLP) using the bovine growth hormone (GH) gene as a probe. This and further analysis of selected DNA samples with 6 additional restriction enzymes (EcoRV Pst I, PvuII, Rsal, Sacl and TaqI) revealed RFLP’s that is probably due to the presence of two alleles at the GH locus; A predominant allele (Gh2), in which the growth hormone gene is duplicated and the two copies are located at a distance of 3.5 Kb apart, and a rare allele (Ghl), in which the growth hormone gene is present as a single copy. The results also indicate that while the 5’ flanking regions of the two GH copies of the Gh2 allele are different, the 3’ flanking region appears to be conserved. © 1990, Taylor & Francis Group, LLC. All rights reserved.
Scientific Publication
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