חיפוש מתקדם
Animal Biotechnology
Valinskv, A., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Gootwine, E., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
DNA samples from 70 sheep of the Awassi, Assaf, Booroola Merino and German Mutton Merino breeds, of the Booroola Merino X Awassi and Booroola Merino X Assaf crosses, of three wild sheep, and of seven Saanen goats, were digested with the restriction enzymes BamHI, EcoRI and HindIII, and screened for restriction fragment length polymorphism (RFLP) using the bovine growth hormone (GH) gene as a probe. This and further analysis of selected DNA samples with 6 additional restriction enzymes (EcoRV Pst I, PvuII, Rsal, Sacl and TaqI) revealed RFLP’s that is probably due to the presence of two alleles at the GH locus; A predominant allele (Gh2), in which the growth hormone gene is duplicated and the two copies are located at a distance of 3.5 Kb apart, and a rare allele (Ghl), in which the growth hormone gene is present as a single copy. The results also indicate that while the 5’ flanking regions of the two GH copies of the Gh2 allele are different, the 3’ flanking region appears to be conserved. © 1990, Taylor & Francis Group, LLC. All rights reserved.
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הספר "אוצר וולקני"
אודות
תנאי שימוש
Restriction Fragment Length Polymorphism In Sheep at The Growth Hormone Locus is The Result of Variation In Gene Number
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Valinskv, A., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Gootwine, E., Institute of Animal Science, A.R.O. The Volcani Center, Bet Dagan 50250, Israel
Restriction Fragment Length Polymorphism In Sheep at The Growth Hormone Locus is The Result of Variation In Gene Number
DNA samples from 70 sheep of the Awassi, Assaf, Booroola Merino and German Mutton Merino breeds, of the Booroola Merino X Awassi and Booroola Merino X Assaf crosses, of three wild sheep, and of seven Saanen goats, were digested with the restriction enzymes BamHI, EcoRI and HindIII, and screened for restriction fragment length polymorphism (RFLP) using the bovine growth hormone (GH) gene as a probe. This and further analysis of selected DNA samples with 6 additional restriction enzymes (EcoRV Pst I, PvuII, Rsal, Sacl and TaqI) revealed RFLP’s that is probably due to the presence of two alleles at the GH locus; A predominant allele (Gh2), in which the growth hormone gene is duplicated and the two copies are located at a distance of 3.5 Kb apart, and a rare allele (Ghl), in which the growth hormone gene is present as a single copy. The results also indicate that while the 5’ flanking regions of the two GH copies of the Gh2 allele are different, the 3’ flanking region appears to be conserved. © 1990, Taylor & Francis Group, LLC. All rights reserved.
Scientific Publication
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