Kathiravan, K., Department of Virology, Institute of Plant Protection, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel, Department of Biotechnology, University of Madras, Guindy Campus, Chennai, 600 025, India
Vengedesan, G., Department of Food Science, Institute of Storage and Food Technology, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel, Hardwood Tree Improvement and Regeneration Center, Purdue University, Department of Forestry and Natural Resources, 715 West State Street, West Lafayette, IN 47907-2061, United States
Singer, S., Department of Virology, Institute of Plant Protection, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Steinitz, B., Department of Vegetable Crops and Plant Genetics, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Paris, H.S., Department of Vegetable Crops and Plant Genetics, Institute of Field and Garden Crops, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay, 30095, Israel
Gaba, V., Department of Virology, Institute of Plant Protection, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Cucurbita pepo L. (squash, pumpkin) is a highly polymorphic vegetable species of major importance. Our study characterized a spectrum of C. pepo germplasm for the ability to regenerate in vitro by direct organogenesis from cotyledon explants. Cultivars tested included both cultivated subspecies, texana and pepo, and nearly all of their respective cultivar-groups. Direct shoot regeneration occurred in all accessions, and was generally high (56-94%), with a single exception of 22% ('Bolognese'). There was no significant difference between the percentage regeneration of the two subspecies. Shoot regeneration per responding explant was uniform (1.2-1.6 shoots per explant). Only 'True French' produced statistically more shoots (3.9 per explant) than other accessions. The morphology of regeneration varied. Most cultivars produced long shoots, often fasciated, amid a few small buds. Some subspecies pepo cultivars (Beirut, Yugoslavia 7, Ma'yan and True French) produced short, massive, hollow shoots, sometimes accompanied by shoots that were more normal. Two subspecies texana cultivars (Creamy Straightneck and Small Bicolor) produced single (sometimes double) shoots without other buds. The production of chimeric (mixoploid) regenerants varied and there was a tendency to regenerate chimeric plants from the widest-fruited accessions (i.e. lowest length-to-width ratio) in each subspecies. Subspecies pepo Pumpkin Group 'Tondo di Nizza' showed significantly greater production of chimeric regenerants. In comparison with the great range of variation observed in fruit shape, the variation of in vitro responses (mostly less than 2-fold in regeneration and shoot production) was less than expected. © Springer 2006.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Adventitious regeneration in vitro occurs across a wide spectrum of squash (Cucurbita pepo) genotypes
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Kathiravan, K., Department of Virology, Institute of Plant Protection, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel, Department of Biotechnology, University of Madras, Guindy Campus, Chennai, 600 025, India
Vengedesan, G., Department of Food Science, Institute of Storage and Food Technology, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel, Hardwood Tree Improvement and Regeneration Center, Purdue University, Department of Forestry and Natural Resources, 715 West State Street, West Lafayette, IN 47907-2061, United States
Singer, S., Department of Virology, Institute of Plant Protection, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Steinitz, B., Department of Vegetable Crops and Plant Genetics, Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Paris, H.S., Department of Vegetable Crops and Plant Genetics, Institute of Field and Garden Crops, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay, 30095, Israel
Gaba, V., Department of Virology, Institute of Plant Protection, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Adventitious regeneration in vitro occurs across a wide spectrum of squash (Cucurbita pepo) genotypes
Cucurbita pepo L. (squash, pumpkin) is a highly polymorphic vegetable species of major importance. Our study characterized a spectrum of C. pepo germplasm for the ability to regenerate in vitro by direct organogenesis from cotyledon explants. Cultivars tested included both cultivated subspecies, texana and pepo, and nearly all of their respective cultivar-groups. Direct shoot regeneration occurred in all accessions, and was generally high (56-94%), with a single exception of 22% ('Bolognese'). There was no significant difference between the percentage regeneration of the two subspecies. Shoot regeneration per responding explant was uniform (1.2-1.6 shoots per explant). Only 'True French' produced statistically more shoots (3.9 per explant) than other accessions. The morphology of regeneration varied. Most cultivars produced long shoots, often fasciated, amid a few small buds. Some subspecies pepo cultivars (Beirut, Yugoslavia 7, Ma'yan and True French) produced short, massive, hollow shoots, sometimes accompanied by shoots that were more normal. Two subspecies texana cultivars (Creamy Straightneck and Small Bicolor) produced single (sometimes double) shoots without other buds. The production of chimeric (mixoploid) regenerants varied and there was a tendency to regenerate chimeric plants from the widest-fruited accessions (i.e. lowest length-to-width ratio) in each subspecies. Subspecies pepo Pumpkin Group 'Tondo di Nizza' showed significantly greater production of chimeric regenerants. In comparison with the great range of variation observed in fruit shape, the variation of in vitro responses (mostly less than 2-fold in regeneration and shoot production) was less than expected. © Springer 2006.
Scientific Publication