Yermiyahu, U., Institute of Soil, Water Environ. Sci., Agric. Res. O., Bet Dagan, Israel Nir, S., Seagram Ctr. for Soil and Water Sci., Fac. Agric., Hebrew Univ. J., Rehovot, Israel Ben-Hayyim, G., Institute of Horticulture, Agric. Res. Org., Volcani C., Bet Dagan, Israel Kafkafi, U., Dept. of Field and Vegetable Crops, Fac. Agric., Hebrew Univ. J., Rehovot, Israel Scherer, G.F.E., Institute for Ornamentals, Tree Appl. Genet., Univ. H., Hannover, Germany Kinraide, T.B., Appalachian Soil Water Conserv. R., Agric. Res. Serv., US Dept. Agric., Beaver, WV 25813-0400, United States
The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca2+ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of 45Ca2+ to the vesicles in the presence of varying concentrations of Ca2+, Na+ and Mg2+. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca2+, Na+ and Mg2+ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M-1, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca2+ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca2+-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance. Copyright (C) 1999 Elsevier Science B.V.
Surface properties of plasma membrane vesicles isolated from melon (Cucumus melo L.) root cells differing in salinity tolerance
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Yermiyahu, U., Institute of Soil, Water Environ. Sci., Agric. Res. O., Bet Dagan, Israel Nir, S., Seagram Ctr. for Soil and Water Sci., Fac. Agric., Hebrew Univ. J., Rehovot, Israel Ben-Hayyim, G., Institute of Horticulture, Agric. Res. Org., Volcani C., Bet Dagan, Israel Kafkafi, U., Dept. of Field and Vegetable Crops, Fac. Agric., Hebrew Univ. J., Rehovot, Israel Scherer, G.F.E., Institute for Ornamentals, Tree Appl. Genet., Univ. H., Hannover, Germany Kinraide, T.B., Appalachian Soil Water Conserv. R., Agric. Res. Serv., US Dept. Agric., Beaver, WV 25813-0400, United States
Surface properties of plasma membrane vesicles isolated from melon (Cucumus melo L.) root cells differing in salinity tolerance
The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca2+ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of 45Ca2+ to the vesicles in the presence of varying concentrations of Ca2+, Na+ and Mg2+. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca2+, Na+ and Mg2+ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M-1, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca2+ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca2+-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance. Copyright (C) 1999 Elsevier Science B.V.