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פותח על ידי קלירמאש פתרונות בע"מ -
Surface properties of plasma membrane vesicles isolated from melon (Cucumus melo L.) root cells differing in salinity tolerance
Year:
1999
Authors :
בן חיים, גוזל
;
.
ירמיהו, אורי
;
.
Volume :
14
Co-Authors:
Yermiyahu, U., Institute of Soil, Water Environ. Sci., Agric. Res. O., Bet Dagan, Israel
Nir, S., Seagram Ctr. for Soil and Water Sci., Fac. Agric., Hebrew Univ. J., Rehovot, Israel
Ben-Hayyim, G., Institute of Horticulture, Agric. Res. Org., Volcani C., Bet Dagan, Israel
Kafkafi, U., Dept. of Field and Vegetable Crops, Fac. Agric., Hebrew Univ. J., Rehovot, Israel
Scherer, G.F.E., Institute for Ornamentals, Tree Appl. Genet., Univ. H., Hannover, Germany
Kinraide, T.B., Appalachian Soil Water Conserv. R., Agric. Res. Serv., US Dept. Agric., Beaver, WV 25813-0400, United States
Facilitators :
From page:
237
To page:
249
(
Total pages:
13
)
Abstract:
The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca2+ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of 45Ca2+ to the vesicles in the presence of varying concentrations of Ca2+, Na+ and Mg2+. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca2+, Na+ and Mg2+ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M-1, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca2+ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca2+-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance. Copyright (C) 1999 Elsevier Science B.V.
Note:
Related Files :
artificial membrane
hypothesis
Magnesium
plant
root
salinity
Surface charge
surface property
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0927-7765(99)00039-9
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31568
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:03
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Scientific Publication
Surface properties of plasma membrane vesicles isolated from melon (Cucumus melo L.) root cells differing in salinity tolerance
14
Yermiyahu, U., Institute of Soil, Water Environ. Sci., Agric. Res. O., Bet Dagan, Israel
Nir, S., Seagram Ctr. for Soil and Water Sci., Fac. Agric., Hebrew Univ. J., Rehovot, Israel
Ben-Hayyim, G., Institute of Horticulture, Agric. Res. Org., Volcani C., Bet Dagan, Israel
Kafkafi, U., Dept. of Field and Vegetable Crops, Fac. Agric., Hebrew Univ. J., Rehovot, Israel
Scherer, G.F.E., Institute for Ornamentals, Tree Appl. Genet., Univ. H., Hannover, Germany
Kinraide, T.B., Appalachian Soil Water Conserv. R., Agric. Res. Serv., US Dept. Agric., Beaver, WV 25813-0400, United States
Surface properties of plasma membrane vesicles isolated from melon (Cucumus melo L.) root cells differing in salinity tolerance
The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca2+ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of 45Ca2+ to the vesicles in the presence of varying concentrations of Ca2+, Na+ and Mg2+. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca2+, Na+ and Mg2+ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M-1, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca2+ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca2+-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance. Copyright (C) 1999 Elsevier Science B.V.
Scientific Publication
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