אסיף מאגר המחקר החקלאי

Identifying Lychee (Litchi chinensis Sonn.) Cultivars and their Genetic Relationships Using Intersimple Sequence Repeat (ISSR) Markers

Year:

2003

Source of publication :

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Volume :

128

Co-Authors:

Degani, C., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel, Guangxi Polytech Institute, Mingyuang, Nanning, Guangxi, China
Deng, J., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel
Beiles, A., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel, Institute of Evolution, Univ. of Haifa, Haifa 31905, Israel
El-Batsri, R., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel, Guangxi Polytech Institute, Mingyuang, Nanning, Guangxi, China
Goren, M., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel, Guangxi Polytech Institute, Mingyuang, Nanning, Guangxi, China
Gazit, S., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel, Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, Rehovot 76100, Israel

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Abstract:

There is widespread confusion and uncertainty concerning the identity of lychee cultivars: the same cultivar may be known under different names and different cultivars may appear under the same name. In the present study, the potential of intersimple sequence repeat (ISSR) for the identification of 66 lychee cultivars and accessions and a determination of their genetic relationships was evaluated, using 32 primers containing different simple sequence repeat motifs. Of the 194 bands produced, 124 (64%) were polymorphic. A set of six ISSR primers was sufficient to distinguish all cultivars and accessions. Thus, cultivars which are morphologically very similar and have identical isozyme profiles can be distinguished by ISSR analysis. However, seven pairs of accessions, each considered to be the same cultivar, were found to be identical by ISSR analysis. Nei and Li band-sharing distances and Nei genetic distances were calculated among the cultivars and two similarity dendrograms were generated using the neighbor-joining algorithm. Results showed that the ISSR technique is a valuable tool for identification of lychee cultivars and analysis of their genetic relationships.

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