חיפוש מתקדם
Spiegel, S., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Scott, S.W., Department of Plant Pathology and Physiology, Clemson University, Clemson, SC, United States
Bowman-Vance, V., Dept. of Biological Sciences, University of South Carolina, Columbia, SC, United States
Tam, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Galiakparov, N.N., Institute of Molecular Biology and Biochemistry, National Center of Biotechnology, Almaty, Kazakhstan
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
The reverse transcription-polymerase chain reaction (RT-PCR) technique was used for detection of prunus necrotic ringspot virus in dormant peach trees which tested negative by ELISA. Total RNA extracted from bark tissue, using a lithium chloride based method, were used for reverse transcription and subsequent amplification of vital sequences. The PCR product, about 300 base pairs in size, was analyzed by gel electrophoresis and visualized by ethidium bromide staining. In some cases, PCR products were not clearly visible in the stained gel and became distinct only following hybridisation with a 32P-labelled virus specific probe. The RT-PCR assay described in this paper is sensitive enough for detection of PNRSV in dormant woody bark tissue and could be incorporated into testing protocols during post-entry quarantines for rapid initial screening of imported budwood and in virus elimination programs.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction
102
Spiegel, S., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Scott, S.W., Department of Plant Pathology and Physiology, Clemson University, Clemson, SC, United States
Bowman-Vance, V., Dept. of Biological Sciences, University of South Carolina, Columbia, SC, United States
Tam, Y., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Galiakparov, N.N., Institute of Molecular Biology and Biochemistry, National Center of Biotechnology, Almaty, Kazakhstan
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction
The reverse transcription-polymerase chain reaction (RT-PCR) technique was used for detection of prunus necrotic ringspot virus in dormant peach trees which tested negative by ELISA. Total RNA extracted from bark tissue, using a lithium chloride based method, were used for reverse transcription and subsequent amplification of vital sequences. The PCR product, about 300 base pairs in size, was analyzed by gel electrophoresis and visualized by ethidium bromide staining. In some cases, PCR products were not clearly visible in the stained gel and became distinct only following hybridisation with a 32P-labelled virus specific probe. The RT-PCR assay described in this paper is sensitive enough for detection of PNRSV in dormant woody bark tissue and could be incorporated into testing protocols during post-entry quarantines for rapid initial screening of imported budwood and in virus elimination programs.
Scientific Publication
You may also be interested in