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פותח על ידי קלירמאש פתרונות בע"מ -
Hydrodynamics based transfection in normal and fibrotic rats
Year:
2006
Source of publication :
World Journal of Gastroenterology
Authors :
פינס, מרק
;
.
Volume :
12
Co-Authors:
Yeikilis, R., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Gal, S., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Kopeiko, N., Department of Experimental Surgery, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Paizi, M., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Pines, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Braet, F., Australian Key Centre for Microscopy and Microanalysis, The University of Sydney, Sydney, NSW 2006, Australia
Spira, G., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Facilitators :
From page:
6149
To page:
6155
(
Total pages:
7
)
Abstract:
Aim: Hydrodynamics based transfection (HBT), the injection of a large volume of naked plasmid DNA in a short time is a relatively simple, efficient and safe method for in vivo transfection of liver cells. Though used for quite some time, the mechanism of gene transfection has not yet been elucidated. Methods: A luciferase encoding plasmid was injected using the hydrodynamics based procedure into normal and thioacetamide-induced fibrotic Sprague Dawley rats. Scanning and transmission electron microscopy images were taken. The consequence of a dual injection of Ringer solution and luciferase pDNA was followed. Halofuginone, an anti collagen type I inhibitor was used to reduce ECM load in fibrotic rats prior to the hydrodynamic injection. Results: Large endothelial gaps formed as soon as 10′ following hydrodynamic injection; these gradually returned to normal 10 d post injection. Hydrodynamic administration of Ringer 10 or 30 m prior to moderate injection of plasmid did not result in efficient transfection suggesting that endothelial gaps by themselves are not sufficient for gene expression. Gene transfection following hydrodynamic injection in thioacetamide induced fibrotic rats was diminished coinciding with the level of fibrosis. Halofuginone, a specific collagen type I inhibitor, alleviated this effect. Conclusion: The hydrodynamic pressure formed following HBT results in the formation of large endothelial gaps. These gaps, though important in the transfer of DNA molecules from the blood to the space of Disse are not enough to provide the appropriate conditions for hepatocyte transfection. Hydrodynamics based injection is applicable in fibrotic rats provided that ECM load is reduced. © 2006 The WJG Press. All rights reserved.
Note:
Related Files :
animal experiment
animal model
Animals
dna transfection
Endothelial lining
Fibrosis
gene expression
liver sinusoid
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31629
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:04
Scientific Publication
Hydrodynamics based transfection in normal and fibrotic rats
12
Yeikilis, R., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Gal, S., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Kopeiko, N., Department of Experimental Surgery, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Paizi, M., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Pines, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Braet, F., Australian Key Centre for Microscopy and Microanalysis, The University of Sydney, Sydney, NSW 2006, Australia
Spira, G., Department of Anatomy and Cell Biology, The Bruce Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, POB 9649, Technion, Haifa 31096, Israel
Hydrodynamics based transfection in normal and fibrotic rats
Aim: Hydrodynamics based transfection (HBT), the injection of a large volume of naked plasmid DNA in a short time is a relatively simple, efficient and safe method for in vivo transfection of liver cells. Though used for quite some time, the mechanism of gene transfection has not yet been elucidated. Methods: A luciferase encoding plasmid was injected using the hydrodynamics based procedure into normal and thioacetamide-induced fibrotic Sprague Dawley rats. Scanning and transmission electron microscopy images were taken. The consequence of a dual injection of Ringer solution and luciferase pDNA was followed. Halofuginone, an anti collagen type I inhibitor was used to reduce ECM load in fibrotic rats prior to the hydrodynamic injection. Results: Large endothelial gaps formed as soon as 10′ following hydrodynamic injection; these gradually returned to normal 10 d post injection. Hydrodynamic administration of Ringer 10 or 30 m prior to moderate injection of plasmid did not result in efficient transfection suggesting that endothelial gaps by themselves are not sufficient for gene expression. Gene transfection following hydrodynamic injection in thioacetamide induced fibrotic rats was diminished coinciding with the level of fibrosis. Halofuginone, a specific collagen type I inhibitor, alleviated this effect. Conclusion: The hydrodynamic pressure formed following HBT results in the formation of large endothelial gaps. These gaps, though important in the transfer of DNA molecules from the blood to the space of Disse are not enough to provide the appropriate conditions for hepatocyte transfection. Hydrodynamics based injection is applicable in fibrotic rats provided that ECM load is reduced. © 2006 The WJG Press. All rights reserved.
Scientific Publication
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