נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Overexpression of UV-DAMAGED DNA BINDING PROTEIN 1 links plant development and phytonutrient accumulation in high pigment-1 tomato
Year:
2010
Source of publication :
Journal of Experimental Botany
Authors :
אבנור, דליה
;
.
חן, לאה
;
.
לוין, אילן
;
.
נהון, סעדיה
;
.
עזרי, רביב
;
.
ראובני, משה
;
.
שלמה, חביבה
;
.
Volume :
61
Co-Authors:
Azari, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Reuveni, M., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Evenor, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Nahon, S., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Shlomo, H., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Chen, L., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Levin, I., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
3627
To page:
3637
(
Total pages:
11
)
Abstract:
Fruits of tomato plants carrying the high pigment-1 mutations hp-1 and hp-1w are characterized by an increased number of plastids coupled with enhanced levels of functional metabolites. Unfortunately, hp-1 mutant plants are also typified by light-dependent retardation in seedling and whole-plant growth and development, which limits their cultivation. These mutations were mapped to the gene encoding UV-DAMAGED DNA BINDING PROTEIN 1 (DDB1) and, recently, fruit-specific RNA interference studies have demonstrated an increased number of plastids and enhanced carotenoid accumulation in the transgenic tomato fruits. However, whole-plant overexpression of DDB1, required to substantiate its effects on seedling and plant development and to couple them with fruit phenotypes, has heretofore been unsuccessful. In this study, five transgenic lines constitutively overexpressing normal DDB1 in hp-1 mutant plants were analysed. Eleven-day-old seedlings, representing these lines, displayed up to ∼73-and ∼221-fold overexpression of the gene in hypocotyls and cotyledons, respectively. This overexpression resulted in statistically significant reversion to the non-mutant developmental phenotypes, including more than a full quantitative reversion. This reversion of phenotypes was generally accompanied by correlated responses in chlorophyll accumulation and altered expression of selected light signalling genes: PHYTOCHROME A, CRYPTOCHROME 1, ELONGATED HYPOCOTYL 5, and the gene encoding CHLOROPHYLL A/B-BINDING PROTEIN 4. Cumulatively, these results provide the missing link between DDB1 and its effects on tomato plant development. © 2010 The Author.
Note:
Related Files :
chlorophyll
gene expression
Genetics
mutation
phenotype
plant growth
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/erq176
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31667
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:04
You may also be interested in
Scientific Publication
Overexpression of UV-DAMAGED DNA BINDING PROTEIN 1 links plant development and phytonutrient accumulation in high pigment-1 tomato
61
Azari, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Reuveni, M., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Evenor, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Nahon, S., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Shlomo, H., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Chen, L., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Levin, I., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Overexpression of UV-DAMAGED DNA BINDING PROTEIN 1 links plant development and phytonutrient accumulation in high pigment-1 tomato
Fruits of tomato plants carrying the high pigment-1 mutations hp-1 and hp-1w are characterized by an increased number of plastids coupled with enhanced levels of functional metabolites. Unfortunately, hp-1 mutant plants are also typified by light-dependent retardation in seedling and whole-plant growth and development, which limits their cultivation. These mutations were mapped to the gene encoding UV-DAMAGED DNA BINDING PROTEIN 1 (DDB1) and, recently, fruit-specific RNA interference studies have demonstrated an increased number of plastids and enhanced carotenoid accumulation in the transgenic tomato fruits. However, whole-plant overexpression of DDB1, required to substantiate its effects on seedling and plant development and to couple them with fruit phenotypes, has heretofore been unsuccessful. In this study, five transgenic lines constitutively overexpressing normal DDB1 in hp-1 mutant plants were analysed. Eleven-day-old seedlings, representing these lines, displayed up to ∼73-and ∼221-fold overexpression of the gene in hypocotyls and cotyledons, respectively. This overexpression resulted in statistically significant reversion to the non-mutant developmental phenotypes, including more than a full quantitative reversion. This reversion of phenotypes was generally accompanied by correlated responses in chlorophyll accumulation and altered expression of selected light signalling genes: PHYTOCHROME A, CRYPTOCHROME 1, ELONGATED HYPOCOTYL 5, and the gene encoding CHLOROPHYLL A/B-BINDING PROTEIN 4. Cumulatively, these results provide the missing link between DDB1 and its effects on tomato plant development. © 2010 The Author.
Scientific Publication
You may also be interested in