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Jeong, D.H., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
German, M.A., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Rymarquis, L.A., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Thatcher, S.R., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Green, P.J., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
MicroRNAs (miRNAs) are small regulatory noncoding RNAs varying in length between 20 and 24 nucleotides. They play a key role during plant development by negatively regulating gene expression at the posttranscriptional level. Moreover, recent studies reported several miRNAs associated with abiotic stress responses. Small RNA cloning and high-throughput deep sequencing methods provide expression profiles of not only known miRNAs, but also novel miRNAs. In this chapter, we describe the methods used to identify and characterize abiotic stress-associated miRNAs and their target genes.
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תנאי שימוש
Abiotic stress-associated miRNAs: detection and functional analysis.
592
Jeong, D.H., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
German, M.A., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Rymarquis, L.A., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Thatcher, S.R., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Green, P.J., Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Abiotic stress-associated miRNAs: detection and functional analysis.
MicroRNAs (miRNAs) are small regulatory noncoding RNAs varying in length between 20 and 24 nucleotides. They play a key role during plant development by negatively regulating gene expression at the posttranscriptional level. Moreover, recent studies reported several miRNAs associated with abiotic stress responses. Small RNA cloning and high-throughput deep sequencing methods provide expression profiles of not only known miRNAs, but also novel miRNAs. In this chapter, we describe the methods used to identify and characterize abiotic stress-associated miRNAs and their target genes.
Scientific Publication
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